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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein | ||
| Clone | 4D10 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue | ||
| Negative control | 293T cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.  Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.  Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.  Detection of Human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (3 µg/ml). Detection: collorimetric (DAB).  Detection of Human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa Cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence). |
