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EMD Millipore
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25 May 2012
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Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.
Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections
Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.
Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections
Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | 5-methylcytosine conjugated to ovalbumin | ||
| Clone | 162 33 D3 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.4. | ||
| Species reactivity | a broad range of species | ||
| Preservative | None | ||
| Applications |
|
||
| Avoid freeze/thaw | Yes | ||
| Positive control | NIH3T3 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB  Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine. |
