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176902  Anthrax Lethal Factor Protease Substrate, Fluorogenic

Anthrax LF Protease Substrate, Fluorogenic, Bacillus anthracis LF Protease Substrate, Fluorogenic, (Cou)Consensus(K(QSY-35)GG)-NH2

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25 May 2012

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An internally-quenched fluorogenic 19-mer peptide substrate derived from the MAP kinase kinase (MKK) substrate motif. Acts as a sensitive substrate for rapid monitoring of Anthrax lethal factor (LF) protease activity. Also suited for simple enzymatic screening of inhibitors in a multiwell plate-based format. Substrate is cleaved between Pro8-Ile9 with a 9-16% increase in fluorescence (at 100% cleavage).
Product information
Peptide Sequence (Cou)-N-Nle-Lys-Lys-Lys-Lys-Val-Leu-Pro-Ile-Gln-Leu-Asn-Ala-Ala-Thr-Asp-Lys-(QSY-35)-Gly-Gly-NH2 Cou = 7-Hydroxy-4-methyl-3-acetylcoumarinyl; QSY-35 = N-((4-((7-Nitro-2,1,3-benzoxzdiazol-4-yl)amino)phenyl)acetyl
Form Red-orange lyophilized solid
Formulation Supplied as a trifluoroacetate salt.
Molar mass 2519.9
Protect from Light Yes
Hygrocopic Yes
Packaged under inert gas Yes
Purity ≥98% by HPLC
Solubility DMSO
Chemical formula C116H179N31O32
Store and ship information
Storage -20°C
Ship Blue Ice Only
Standard Handling

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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