For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
22 May 2013
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Optimized for flow cytometry and fluorescence microscopy applications. A RAPID™ protocol has been developed for Annexin V-FITC binding directly in tissue culture media. This obviates the need for tedious centrifugation and wash steps, that increase the occurrence of mechanical membrane disruption. In addition, since apoptosis is a dynamic process that is ongoing once cells are removed from culture conditions and continues throughout experimental processing, the RAPID™ protocol is recommended for the detection of cells in early apoptosis.
| Product information | |||
|---|---|---|---|
| Format | Flow cytometry or fluorescence microscopy | ||
| Form | 100 Tests | ||
| Detection method | Fluorometric | ||
| Species reactivity | a broad range of species | ||
| Applications |
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| Do not freeze | Yes | ||
| Assay time | 15 - 30 min | ||
| Sample type | Intact cells | ||
| Positive control | Apoptotic Jurkat cells (dexamethasone or anti-Fas treated) | ||
| Kit contains | Annexin V-FITC, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol. | ||
| Declaration | Sold under license of U.S. Patent 5,834,196. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-24 Do not breathe dust. Avoid contact with skin. |
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| R Phrase |
R: 36 Irritating to eyes. |
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