176871
Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells
Peptidyl-Dipetidase A, ACE
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl2, 40% glycerol, pH 7.5. | ||
| Molar mass | 120000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥1 unit/µg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5. | ||
| Purity | ≥95% by SDS-PAGE | ||
| Contaminants | Endotoxins: ≤1.0 EU/µg ACE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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