171539
AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda
AMP Kinase (α2/β1/γ1)
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EMD Millipore
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25 May 2012
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Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5. | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥270 nmol/min/mg protein | ||
| Unit definition | Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP. | ||
| Purity | ≥75% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 The activity of AMPK (α2/β1/γ1) was measured as outlined in the Recommended Reaction Conditions. |
