Method Transfer from TLC or HPTLC to HPLC Column
EMD has specialized the manufacturing of standardized silica gel packing materials for TLC, analytical and as well as preparative chromatography and offers a wide range of customized sorbents.
Main advantages of TLC and HPTLC:
- analysis of oils or fat and other complex sample matrices is possible without sample preparation
- multiple samples (up to 40) can be run simultaneously under identical conditions
- faster and cheaper separation methods are possible without the need for sophisticated instrumentation
- easy 2-dimensional separation by using two distinct mobile phases in different directions gives unique separation quality
Because of this, identical raw material is available for stationary phases for both TLC and HPLC, resulting in equivalent chromatographic selectivity for both techniques (see fig. 1)
Fig. 1: TLC separation (left) and the corresponding HPLC separation (right)
Thin Layer chromatography (TLC) is one of the most versatile separation techniques in pharma, food and cosmetic industry and is also used for fast method development for HPLC.
EMD HPTLC plates offer higher speed and higher sensitivity compared to classical TLC and provide excellent separations. Using instrumental equipment, HPTLC plates enable modern, quantitative thin layer chromatography, which is comparable with HPLC performance (see fig. 1 and 2).
HPTLC and HPLC separations show the same separation results, if both techniques are used under similar chromatographic conditions.
e.g.
- identical sorbents
- identical mobile phases
Separation of real sample (Lettuce) using TLC and HPLC same chromatographic condition
| HPTLC | HPLC |
|---|---|
 |
 |
Fig. 2: separation of lettuce (real sample) HPTLC and HPLC under chromatographic condition [ Dinocap (1), Parathion (2), Iprodon (3),Mathylparathion (4), Metobromuron (5) and Dimethoat (6)]
Sample Preparation: extraction with acetone after crushing lettuce leaves
Chromatographic conditions:
a) HPTLC plate: HPTLC-Silica gel 60 RP-18 F254s
b) Column: LiChroCART® LiChrospher® 100 RP-18
For both methods, the mobile phase is : acetonitrile /water 70/30
Would you like more information? Please contact us or browse our Chromatography Manual.
