Separation of explosives from drinking water
| LiChroCART® 250-4 Purospher® RP-18 HC, 5 μm | ||
|---|---|---|
| Mobile phase | A: Acetonitrile / Methanol; 20 / 80, v/v B: Sodium dihydrogenphosphate buffer (c = 0.01 mol/L, pH 4.5) Gradient 0 min 35 % A; 28 min 55 % A; 40 min 85 % A; 50 min 85 % A; 51 min 35 % A; 71 min 35 % A |
|
| Flow rate | 0.8 mL/min | |
| Detection | DAD 210 and 235 nm | |
| Temperature | 36 °C | |
| In. volume | 40 μL | |
| Sample |
1. Octogen (HMX) |
 |
| Sample preparation | |
|---|---|
| Solid phase extraction with LiChrolut® EN (200 mg) (Cat. No. 1.19870): | |
| Solvents | A: Methanol (LiChrosolv® Cat. No. 1.06007), B: Acetonitrile (LiChrosolv® Cat. No. 1.00030); C: Water (LiChrosolv® Cat. No. 1.15333). |
| Sample pretreatment | Filtrate if necessary and add approx. 5 g NaCl / 1L water sample. Conditioning of extraction column: 3 mL A, 3 mL B, 10 mL C, do not allow column to dry out! |
| Sample application | pass 1 L water sample through the extraction column within 1 hour by using LiChrolut® extraction unit (Cat. No. 1.19851) which is connected to the water sample reservoir with PTFE hose (Cat. No. 1.22143) and a steel capillary. |
| Drying step | 10 min by means of nitrogen and LiChrolut® drying unit (Cat. No. 1.19852). |
| Elution step | 1 x 2 mL, then 1 x 3 mL A/B (1:1), collect in a conical flask and gently evaporate the solvent by means of nitrogen up to a and volume of 0.5 mL. Fill up to 1.0 mL with C. Subsequently filtrate sample into a 1.5 mL sample vial through a 0.2 μm membrane filter. |
