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Scientific Papers on working with SeQuant® ZIC®-HILIC

SeQuant® ZIC®-HILIC HPLC columns solve research problems

Scientific papers on working with Merck’s SeQuant® ZIC®-HILIC

Scientists in laboratories around the world have published articles after solving difficult analysis problems using SeQuant® ZIC®-HILIC technology. Perhaps their experience can help to further your own research by providing examples of specific separation conditions for a particular compound type.
Although we do our best to regularly update this application collection, we would appreciate your help.

 

 

Chiritaa and his colleagues conclude that zwitterionic HILIC columns (they tested SeQuant® ZIC®-HILIC column 150x4.6mm, 5µm, 200Å, cat. no. 150455) are the only types of HILIC columns that are suitable for all types of hydrophilic analytes (anionic, cationic, neutral, and zwitterionic) or as they write:

"On a practical note, these results can be used as follows:
(i) When analyzing anionic compounds, a cationic-type column (amino or triazole) or zwitterionic (ZIC-HILIC) phase should be selected first to provide sufficient retention. In the optimization process, changing one column for another can be advantageous to modify selectivities, and even sometimes elution order.
(ii) When analyzing cationic compounds, a neutral (cyano, diol or amide), anionic-type (silica gel) or zwitterionic phase (ZIC-HILIC) should be selected to achieve sufficient retention. In the optimization process, changing one column for another, especially
hanging a neutral phase for an anionic phase (or vice versa) can provide different selectivities and/or elution orders.
(iii) When analyzing globally neutral compounds such as zwitterionic amino acids, any HILIC phase is potentially capable of providing sufficient retention. In the optimization process, it is more advantageous to replace a particular stationary phase chemistry by a very different one, to provide different selectivities and elution orders."

Scientific Papers on working with SeQuant® ZIC®-HILIC HPLC columns

Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis.
R.-I. Chiritaa, C. Westa, A.-L. Finarub and C. Elfakir
J. Chromatogr. A. 1217 (2010) 3091-3104

Three most recent scientific papers on working with ZIC®-HILIC:

1. Development of a new method using HILIC-tandem mass spectrometry for the characterization of O-sialoglycopeptides from proteolytically digested caseinomacropeptide.
O. Hernandez-Hernandez, R. Lebron-Aguilar, J.E. Quintanilla-Lopez, M. L. Sanz, F.J. Moreno
Proteomics, 10 (2010) 3699-3711

2. Approach to hydrophilic interaction chromatography column selection: Application to neurotransmitters analysis.
R.-I. Chiritaa, C. Westa, A.-L. Finarub and C. Elfakir
J. Chromatogr. A. 1217 (2010) 3091-3104

3. Metabolomic applications of HILIC-LC-MS.
S. Cubbon, C. Antonio, J. Wilson, J. Thomas-Oates
Mass Spectrom. Rev., 29 (2010) 671-684
  

Visit our on-line tutorial to learn more about HILIC technology or request our comprehensive „A Practical Guide to HILIC“ booklet. Both provide detailed explanations of technology and techniques to ensure your success using EMD Millipore’s innovative SeQuant® ZIC®-HILIC products.

If you still can’t find the information you need or want to publish any novel results of your own, you can always contact the SeQuant® Support Team who are happy to assist.

Click here to find a complete collection of Scientific Papers

Related information

SeQuant® Applications    
  Applications by Topic Glycomics and Glycoproteomics
    Ion Analysis
    Melamine
    Peptide Mapping
    Sugar Analysis
  Application Notes  
  Scientific Papers  
  Technical Notes  

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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