For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 800-645-5476
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
19 June 2013
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Directional PCR cloning into the most powerful E. coli expression vectors
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
| Related products | |
|---|---|
| 69076 | pET-32 Ek/LIC Vector Kit |
| 69077 | pET-30 Ek/LIC Vector Kit |
| 70072 | pET-32 Xa/LIC Vector Kit |
| 70073 | pET-30 Xa/LIC Vector Kit |
| Related products for pET-41 Ek/LIC Vector Kit | |
| 69076 | pET-32 Ek/LIC Vector Kit |
| 69077 | pET-30 Ek/LIC Vector Kit |
| 70072 | pET-32 Xa/LIC Vector Kit |
| 70073 | pET-30 Xa/LIC Vector Kit |
| 71072 | pET-43.1 Ek/LIC Vector Kit |
| 71144 | pET-44 Ek/LIC Vector Kit |
| 71335 | pET-46 Ek/LIC Vector Kit |
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| Vector family | pET | |||||||||||||||||||||||||||||||||||||||||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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