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TLC & HPTLC

Mass spectrometry TLC/HPTLC glass plates

MS TLC/HPTLC glass plates

EMD Millipore has pioneered thin-layer chromatography. EMD Millipore introduced the first pre-coated plates on the market and regularly added new products according to customers’ requirements for planar chromatography applications. 

EMD Millipore, in its role as a pioneer, introduced the first glass plates on the market for coupling planer chromatography with mass spectrometry.
Today, coupling TLC plates with mass spectrometry is a new field of high interest, which will contribute substantially to the progress of planar chromatography – both today and in the future. One particular advantage of TLC-MS is the flexibility in choosing mobile phases for separation, compared to HPLC-MS coupling, where certain mobile phases cannot be used (e.g. inorganic buffers cannot be used with HPLC-MS).

The techniques for coupling TLC with mass spectrometry can be divided into a) elution-based and b) desorption-based techniques. Both approaches are offline, and both are performed after the separation is completed and the plate dried. The sample transfer to the MS is fast and typically takes less than one minute.

  • a ) With elution-based techniques, the analyte on the silica plate is dissolved in a solvent and transferred to the mass spectrometer in the liquid phase.
  • b) With desorption-based techniques, the analyte is vaporized from the silica and transferred to the MS in the gas phase. Vaporization techniques include gas beam, ion bombardment and MALDI (matrix-assisted laser desorption/ionization).

 

  20x20 cm 20x10 cm
TLC silica gel 60 F254 MS-grade 1.00933.0001  
HPTLC silica gel 60 F254 MS-grade   1.00934.0001

 

The separation performance of both new products is equivalent to the standard TLC/HPTLC  plates; the only difference is that the new products are much cleaner than the standard plates and allow trace analysis in the nanogram range.

The following experimental results demonstrate the enhanced sensitivity of TLC-MS using the new plates:

a) LC-MS background signal measurement on an HPTLC silica gel 60 F254 standard plate 20 x 10 cm (1.05642.0001) with mobile phase ACN/water (95/5).

LC-MS with standard plate

b) LC-MS background measurement on an HPTLC silica gel 60 F254 MS-grade plate, 20 x 10 cm (1.00934.0001) with mobile phase ACN/water (95/5).

LC-MS with new HPTLC MS-grade plate

This clearly demonstrates the very low background signal using the MS-grade plate.

 

c) Trace measurement of caffeine on an HPTLC-MS grade plate

Plate: HPTLC silica gel 60 F254 MS-grade 25 glass plates 20x10cm (1.00934.0001)
Sample: 20 ng caffeine (MH+) 195.1
Mobile phase: ACN / water (95/5) + 0.1% formic acid

 Trace measurement of caffeine on an HPTLC-MS grade plate

Fig. C: ESI-MS mass spectrum of caffeine, measured from a 20 nanogram TLC spot

These experiments clearly demonstrate the performance of the NEW MS-grade TLC/HPTLC plates.

We strongly recommend these NEW MS-grade plates for TLC-MS applications.

 

  20x20 cm 20x10 cm
TLC silica gel 60 F254 MS-grade 1.00933.0001  
HPTLC silica gel 60 F254 MS-grade   1.00934.0001

 

TLC & HPTLC    
  TLC  
  HPTLC  
  PLC  
  Special Plates  
    Concentrating zone plates
    GLP plates
    Kieselguhr and mixed layer plates
    MS grade plates
    Multiformat plates
    ProteoChrom® HPTLC plates

© Merck KGaA, Darmstadt, Germany, 2014

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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