InnoCyte™ ECM Cell Adhesion Assay, Galectin-1/Galectin-3
For general questions please contact our Customer Service:
290 Concord Road
01821 Billerica, MA
Phone: 781 533-6000
24 May 2013
This product has been discontinued.
The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.
• 96-well tissue culture plate coated with Galectin-1/Galectin-3
• Assay is quick-can be performed in 2.5 h
• Adherent cells are labeled with calcein-AM
• Relative cell attachment is assessed using a fluorescence plate reader
|Form||72 Tests (36 Gal-1 and 36 Gal-3)|
|Protect from Light||Yes|
|Assay time||2.5 h|
|Sample type||Adherent cultured cells|
|Kit contains||Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol.|
|Store and ship information|
|Storage||+2°C to +8°C|
Blue Ice Only
Multiple Toxicity Values, refer to MSDS
~40,000 cells were added to wells coated with galectin-1, galectin-3, and BSA and incubated for 1.5 h at 37°C in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in the presence of 6% CO2. Fluorescence was measured as indicated in the Detailed Protocol. HT-1080 cells displayed appreciable binding to poly-L-lysine, which served as a positive control (data not shown).