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Chromolith HPLC

Chromolith® NH2 HPLC columns application

Chromolith® NH2 HPLC column

Chromolith® NH2 HPLC columns are made of highly porous monolithic rods of silica with a revolutionary bimodal pore structure. Therefore high separation efficiencies are reached under low back-pressures. Chromolith® NH2 HPLC column have long lifetimes within a pH range of 2.5 to 7.5, high matrix tolerance and speed of analysis.

The major application area for amino phases is the separation of carbohydrates (mono- and disaccharides such as fructose, glucose, sucrose, maltose, and lactose), anions, and organic acids. These columns may be used for normal-phase chromatography, reversed-phase chromatography, and weak anion exchange separations.

 

Ordering Information – Chromolith® NH2 HPLC columns

  50x4.6 mm 25x4.6 mm
Chromolith® SpeedROD NH2  1.52027.0001  
Chromolith® Flash NH2    1.52026.0001

 

Separation examples on Chromolith® Performance NH2 100-4.6 mm
Column Chromolith® Performance NH2,
100-4.6 mm
Separation on Chromolith® Performance NH2
Mobile phase A: 100% Acetonitrile (v/v)
B: 100% Water (v/v)
C: 0.05 M Phosphate buffer pH 4.6 (v/v)
Gradient  

Initial composition:
88% A + 9% B + 5% C 

Change to:
80% A + 10% B + 10% C
Hold                               


for 2 min


 in 1 min
until 10 min

Flow rate 1 mL/min
Pressure 24-28 bar (2.8 MPa, 40696 psi)
Detection Dionex Ultimate 3000 VWD-3400, 2.5 Hz,
response time 0.1s, UV = 265 nm
11 μL flow cell
Temperature 25°C
Injection volume 5 μL
Sample 1. Uracil- -D-arabinofuranoside
2. Uridine
3. Cytosine- -D-arabinofuranoside
4. Cytidine
Separation examples on Chromolith® Performance NH2 100-4.6 mm
Column Chromolith® Performance NH2,
100-4.6 mm
Separation on Chromolith® Performance NH2
Mobile phase A: 100% Acetonitrile (v/v)
B: 100% Water (v/v)
C: 20 mM Phosphate buffer pH 3.0 (v/v)
Isocratic   Initial composition:
85% A / 5% B / 10% C v/v/v isocratic
Flow rate 4 mL/min
Pressure 100 bar (10 MPa,145 psi)
Detection Dionex Ultimate 3000 VWD-3400, 2.5Hz,
response time 0.1s, UV 210 nm
11 μL flow cell
Temperature 40°C
Injection volume 1 μL
Sample 1. Norepinephrine
2. Epinephrine tartrate
3. Dopamine
4. DOPA
5. Norephedrine
6. N-Methylephedrine
Separation examples on Chromolith® Performance NH2 100-4.6 mn
123Column Chromolith® Performance NH2,
100-4.6 mm
Separation on Chromolith® Performance NH2
Mobile phase A: 100% Acetonitrile (v/v)
B: 100% Water (v/v)
Isocratic Initial composition:
75% A / 25% B (v/v) isocratic
Flow rate 1 mL/min
Pressure 11 bar (1.1 MPa,16 psi)
Detection Dionex Ultimate 3000 VWD-3400, 2.5Hz,
response time 0.1s, UV 190 nm
11 μL flow cell
Temperature 23°C
Injection volume 10 μL
Sample 1. Xylose
2. Fructose
3. Glucose
4. Saccharose
Separation examples on Chromolith® Performance NH2 100-4.6 mn
Column Chromolith® Performance NH2,
100-4.6 mm
Separation on Chromolith® Performance NH2
Mobile phase A: 100% Acetonitrile (v/v)
B: 100% Ammonium acetate 100 mM
     pH 6.8 (v/v)
Isocratic Initial composition:
90% A / 10% B (v/v)
Flow rate 4 mL/min
Pressure 108 bar (10.8 MPa,157psi)
Detection Dionex Ultimate 3000 VWD-3400, 2.5Hz,
response time 0.1s, UV 240 nm
11 μL flow
Temperature 25°C
Injection volume 5 μL
Sample 1. Ascorbic acid
2. Dehydroascorbic acid

 

Ordering Information – Chromolith® NH2 HPLC columns

  50x4.6 mm 25x4.6 mm
Chromolith® SpeedROD NH2  1.52027.0001  
Chromolith® Flash NH2    1.52026.0001

 

Further suggested reading 

Chromolith® HPLC  
  Detailed information about Chromolith®
HPLC columns
 
  Chromolith® CapRod®  
  Chromolith® column coupler  
  Chromolith® HPLC FastGradient  
  Chromolith® HPLC guard cartridges  
  Chromolith® NH2  
  Example applications using
Chromolith® NH2 HPLC columns
  Chromolith® Prep  
  Chromolith® RP-8 endcapped  
  Chromolith® RP-18 endcapped  
  Chromolith® RP-18 endcapped HighResolution  
  Chromolith® Si  
  Chromolith® SemiPrep  

© Merck KGaA, Darmstadt, Germany, 2014

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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