Chromolith® HPLC column coupler
Chromolith® Performance RP-18 endcapped columns provide rapid high-quality separation of complex mixtures. But, if necessary, the separation efficiency can be increased by coupling several columns together, using the Chromolith® HPLC column coupler. With the column coupler it is possible to increase the plate count by coupling several columns in series. In this way, columns with a theoretical plate count that is significantly higher compared to any particulate column available are produced. At the same time the producing pressure is kept still well below the HPLC system limit.
This added column performance is the key to solve even very critical separation problems where resolution is the limiting factor. Therefore they are perfect for use to separate hitherto non-separable complex mixtures.
The table below shows a comparison between Chromolith® HPLC columns and particulate columns. As you can clearly see, the combination of two Chromolith® Performance RP-18 endcapped columns yields a column with a separation efficiency of 19,000 theoretical plates per column, which is usually the maximum for particulate columns.
Click here to review example applications with Chromolith® Si HPLC columns.
Typical column efficiency using the Chromolith® HPLC column coupler
| Column | Length [mm] | Pressure* [bar] | Plate number per column [Anthracene] |
| Chromolith® Performance 1x | 100 | 30 | 10,000 |
| Chromolith® Performance 2x | 200 | 60 | 19,000 |
| Chromolith® Performance 3x | 300 | 90 | 27,000 |
| Chromolith® Performance 4x | 400 | 120 | 35,000 |
| Chromolith® Performance 5x | 500 | 150 | 41,000 |
| Particulate column (5 μm) | 250 | 220 | 18,500 |
| Particulate column (3.5 μm) | 150 | 400 | 19,000 |
| * = 3 mL/min 75% acetonitrile, 25% water | |||
Ordering Information – Chromolith® HPLC column coupler
| Chromolith® column coupler | 1.51467.0001 |
Further suggested reading
