New Chromolith® HighResolution
The faster way to trouble-free high resolution separations
Revolutionary monolithic silica columns are a single rod of high purity monolithic silica instead of particles. A unique bimodal pore structure gives greatly improved chromatographic performance. This leads mainly to improved separation efficiency and column back-pressure and allows for faster flow rates, considerably reducing the analysis time while enhancing the lifetime.
Order now and get 15% OFF introductory price*!
If you are interested in applications with the new Chromolith® HighResolution please click here.
Benefits of the new Chromolith® HighResolution
- Column performance corresponds to sub 3 µm particle-packed columns and is at least 50% higher compared with our standard Chromolith® columns
- Back-pressure at least half that of particular columns
- Minimum 30% longer column lifetime compared with particle-packed columns
The new Chromolith® HighResolution columns offer higher efficiency and improved peak shape combined with lower back-pressure which is more than two times lower than any particulate packed column with the same dimensions.
Differentiation between Chromolith® and Chromolith® HighResolution
The new Chromolith® HighResolution has at least 50% higher efficiency, excellent peak symmetry and still a more than 30% longer lifetime compared with particulate columns. Two Chromolith® HighResolution columns can easily be coupled in order to achieve even higher resolution. A endcapped stationary phase enables the elution of basic compounds with no peak tailing.
Matrix-rich samples should be analysed with Chromolith® as this type of column will have a longer lifetime. In addition, a lower back-pressure would allow coupling of even more columns if necessary.
| Chromolith® Performance RP-18e, 100-4.6 nm | Chromolith® HighResolution RP-18e, 100-4.6 nm |
||
|---|---|---|---|
| Mobile phase | Acetonitrile/water 60/40 |  |
 |
| Flow rate | 2.0 ml/min | ||
| Detection | UV 254 nm | ||
| Temperature | ambient | ||
| Inj. volume | 5 µl | ||
| Sample | 1. Urea 2. Biphenyl-2-ol 3. Progesterone 4. Hexanophenone 5. Anthracene |
Specification of Chromolith® HighResolution RP-18 endcapped
|
Silica type: |
High-purity |
|
Particle size: |
Monolithic |
|
Macropore size: |
1.15 micrometer |
|
Mesopore size: |
15 nm (150 Angström) |
|
Pore volume: |
1 mL/g |
|
Total pore volume: |
2.9 mL/g |
|
Surface area: |
250 m2/g |
|
Surface modification: |
RP-18 endcapped |
|
Carbon content: |
18 % |
Method transfer from particulate to monolithic Chromolith® columns
Method transfer from particulate to monolithic Chromolith® columns is made easy since the chromatographic selectivity of Chromolith® columns is very similar to that of many modern particulate HPLC columns (e.g. Purospher®). Chromolith® columns are now included in the US Pharmacopoeia in categories L1, L3 and L7.
| 100 mm | 50 mm | 25 mm | 5 mm | |
|---|---|---|---|---|
|
Chromolith® HighResolution RP-18e |
1.52022.0001 | |||
| Chromolith® HighResolution RP-18e 50-4.6 mm ** |
1.52021.0001 | |||
| Chromolith® HighResolution RP-18e 25-4.6 mm ** |
1.52020.0001 | |||
| Chromolith® HighResolution RP-18e 5-4.6 mm Guard Column Kit |
1.52024.0001 | |||
| Chromolith® HighResolution RP-18e 5-4.6 mm Guard Columns (3 pieces) |
1.52025.0001 |
** Products available in 2012
| New Chromolith® HighResolution | |
|---|---|
| Applications featured with Chromolith® HighResolution | |
| Comparison with core-shell material | |
| Speed, packing shape, and reproducibility |
* offer valid till the end of 2011. Prices displayed reflect this additional discount.
