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218791  Caspase-1 Assay Kit, Fluorometric

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EMD Chemicals, Inc.
480 S. Democrat Road
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Phone: 800 854 3417
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18 June 2013

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This kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on detection of the cleavage of the substrate YVAD-AFC. Upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (~505 nm), which can be measured using a fluorometer or a fluorescence microplate reader.
Product information
Format 96-well plate
Form 100 Tests
Detection method Fluorometric
Protect from Light Yes
Assay time 3-3.5 h
Sample type cell lysates
Kit contains Cell Lysis Buffer, Reaction Buffer, YVAD-AFC Substrate, DTT, and a user protocol.
Store and ship information
Storage -20°C
Ship Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 26-36-39-61

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Wear eye/face protection.
Avoid release to the environment. Refer to special instructions/safety data sheet.
R Phrase R: 22-36/38-41-52/53

Harmful if swallowed.
Irritating to eyes and skin.
Risk of serious damage to eyes.
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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