Akt Activity Immunoassay Kit
Protein Kinase B Activity Assay Kit
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
Phone: 800 854 3417
Fax: 800 776 0999
19 May 2013
This product has been discontinued.
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
Note: 1 kit = 40 assays.
|Species reactivity||human, mouse, rat|
|Assay time||4 h|
|Sample type||Cell lysates, tissue extracts|
|Kit contains||Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3α Protein/ATP Mixture, Kinase Assay Buffer, GSK-3α, Phospho-Specific Antibody, and a user protocol.|
|Store and ship information|
Dry Ice Only
Multiple Toxicity Values, refer to MSDS