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124007  Akt Activity Immunoassay Kit

Protein Kinase B Activity Assay Kit

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19 May 2013

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Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates. Note: 1 kit = 40 assays.
Product information
Format Immunoblot
Form 40 Tests
Detection method Immunoblot
Species reactivity human, mouse, rat
Avoid freeze/thaw Yes
Assay time 4 h
Sample type Cell lysates, tissue extracts
Kit contains Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3α Protein/ATP Mixture, Kinase Assay Buffer, GSK-3α, Phospho-Specific Antibody, and a user protocol.
Store and ship information
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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