DDHW00010-WW Direct Detect Spectrometer

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      Replacement Information
      Catalogue NumberDDHW00010-WW
      Trade Name
      • Direct Detect
      DescriptionDirect Detect Spectrometer
      OverviewThe Direct Detect™ infrared (IR)-based quantification system is an innovative combination of software-controlled instrumentation and EMD Millipore Corporation’s advanced membrane technology, optimized for the detection and quantification of proteins. Biomolecules are applied directly to a card-based hydrophilic polytetrafluoroethylene (PTFE) membrane that is transparent in most of the infrared spectral region. The Direct Detect™ system measures amide bonds in protein chains, accurately quantifying an intrinsic component of every protein without relying on amino acid composition, dye binding properties, or redox potential. Protein concentrations from 0.2 to 5 mg/mL can accurately be determined from a minimal sample volume (2 μL) without bio- or immuno-chemical staining. Sample analysis takes only minutes and in most cases can be performed directly from the buffered or native solution.
      Product Information
      • 1 Direct Detect™ Spectrometer
      • 1 Instrument power adapter with 4 power cord/plug configurations (EU, UK, US, Japan)
      • 1 Data cable (crossover cable for 10Base-T Ethernet standard, with RJ45 connectors)
      • 1 Dell® Netbook computer with Direct Detect™ software installed
      • 1 Computer power adapter with world-wide plug adapter
      • 1 Netbook stand
      • Direct Detect™ Assay-free cards (package of 50)
      • 1 Direct Detect™ spotting tray
      • 1 Desiccant pack replacement
      • 1 Torx TX20 screw driver for replacing the IR source
      • 1 Direct Detect™ Spectrometer Quick Start Card
      ApplicationBy measuring amide bonds in protein chains, the Direct Detect Spectrometer system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding or redox potential.
      Key Applications
      • Protein Quantitation
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size1 ea
      Transport Information
      Supplemental Information



      Direct Detect® Spectrometer

      Technical Info

      Fast and accurate peptide quantitation using the Direct Detect® spectrometer
      Guide to choosing the right protein/peptide analysis method for Direct Detect¿ quantitation
      Monitoring detergent removal from biological samples using the Direct Detect® spectrometer
      New, combined lysis and purification reaction simplifies recombinant protein purification with magnetic beads
      Simplified analysis of lipid or detergent content in biological samples using the IR-based Direct Detect® Spectrometer
      The Direct Detect® Biomolecular Quantitation System

      Data Sheet

      Direct Detect™ Spectrometer Data Sheet


      Evaluation of common protein extraction reagents in mammalian and bacteria lysates, by infrared (IR) based quantification and Western blotting
      Methods for the Isolation and Analysis of Biomarkers From Exosomes in Cell Culture Media
      Poster: Novel Condensed Workflow Enabling High Throughput Recombinant Protein Purification from E. coli

      User Guides

      Direct Detect¿ Spectrometer Quick-Start Guide
      Direct Detect¿ Spectrometer Software User Guide
      Direct Detect¿ Spectrometer User Guide