Abstract: The need for ease-of-use and high dosage is driving the use of administration through pre-filled syringes. Ultrafiltration systems are used for formulation and may be challenged to deliver the high viscosities needed.
This webinar highlights the potential limitations of current systems and cassettes and introduces a new high viscosity cassette that can overcome these limitations.
Compacted Media - Improving Solubility and Stability
Topic: In this webinar, we will present data underlining the advantages of compacted media and the stability of raw materials using UPLC and LC-MS methods completed with results from fed-batch cultivation experiments based on compacted cell culture media.
Presenter: Nikolai Stankiewicz, Head of Technology Transfer Laboratory, EMD Millipore
Abstract: Dry powder cell culture media formulations provide many advantages with respect to shipping and storage. However to leverage these benefits, they need to be highly soluble, homogeneous and convenient to handle. An improvement of dry powder media properties can be achieved by the combination of optimized milling procedures and suitable formulation technologies. Starting from a homogeneous dry powder medium, the roller compaction technology is able to fix this homogeneity in larger compactate particles (> 1000 µm) so that de-mixing can be excluded. Additionally, the presented roller compaction offers several other advantages, specifically product stability, based on the fact that only pressure and no water is needed in the production process. Applying media as compactates also reduces dust formation and improves the flowability, making the handling of these cell culture media much more convenient. But the key advancement of using compacted media is the acceleration of the dissolution speed. In this webinar, we will present data underlining the advantages of compacted media and the stability of raw materials using UPLC and LC-MS methods completed with results from fed-batch cultivation experiments based on compacted cell culture media.
New EN ISO 11133 - Quality Assured Culture Media for Food and Water Testing
Topic: Informative webinar on new EN ISO 11133 which is now a full standard and as such mandatory Note: webinar pertains to European ISO standards
Presenter: Barbara Gerten, Application Training Scientist at EMD Millipore and a member of the ISO committee for microbiological standards regarding food and water testing
Abstract: The International Organization for Standardization recently published the revised EN ISO 11133, which is now a full standard and as such mandatory for all accredited laboratories that perform microbiological testing of food, animal feed or water using culture media. It has been completely restructured, clarifying procedures and drawing a clear line between the responsibilities of the laboratories and the manufacturers of culture media regarding media preparation, production, storage and performance testing
Life Science Research
Garbage In = Garbage Out? Preparing Complex Biological Samples for Omics Studies
Topic: How to use infrared-based spectrometry to simultaneously monitor total protein and lipid content during sample preparation. A novel instrument, the Direct Detect® spectrometer, enables anyone, even without spectroscopy training, to exploit IR-based protein quantitation for complex biological samples.
Presenters: Dr. Alexander Lazarev, Ph.D., Vice President of Research and Development at Pressure BioSciences; and Ivona Strug, Ph.D., Senior Biochemical Scientist, EMD Millipore
Analyzing proteomic samples using infrared (IR)-based spectroscopy can be an accurate, information-rich alternative to other assays for total protein. Now, anyone, even without spectroscopy training, can exploit IR-based protein quantitation for even complex biological samples, using the new Direct Detect® spectrometer. In this webinar, you will learn how this intuitive, benchtop system quantitates protein using infrared spectra to measure amide bonds in protein chains, an intrinsic component of every protein. You will also learn how to quickly gauge the reliability of your data with a peek at the shape of the IR spectrum. Finally, even if your protein sample contains detergents, reducing agents and other buffer components that interfere with traditional protein quantitation assays, this video shows you exactly how to set up the Direct Detect® system so that you can still measure protein concentrations accurately.
Goodbye, Bradford Assays! Discover Infrared-Based Protein Quantitation Using the Direct Detect® Spectrometer
Topic: Beginner-level demonstration video shows you exactly how to set up the Direct Detect® system so that you can still measure protein concentrations accurately.
Analyzing protein using infrared (IR) spectrometry is a decades-old technique, and is known to be an accurate, information-rich alternative to other assays for total protein. Now, anyone, even without spectroscopy training, can exploit IR-based protein quantitation for even complex biological samples, using the new Direct Detect® spectrometer.
A Closer Look at Cell Death with Imaging Flow Cytometry
Presenters: Irene La-Beck, Pharm.D., Texas Tech University Health Sciences Center; Sabine Pietkiewicz, Ph.D., Otto von Guericke University, Germany; and Haley R. Pugsley, Ph.D., EMD Millipore
Apoptosis, necrosis, and autophagy are all different forms of programmed cell death (PCD) and are essential for regulating homeostasis and eliminating undesirable cells. Distinguishing between different forms of PCD can be challenging because these processes depend upon changes in morphology and subcellular structure that are often masked in studies of heterogeneous cell populations. The use of imaging flow cytometry has been a method to overcome this problem and has led to new discoveries in the field of PCD.