Abstract: Bioreactor contamination from virus is a persistent threat despite current best practices. Virus detection testing and virus clearance operations may be used in combination to achieve a desired overall risk profile. In this presentation, we discuss new methods for the modeling, quantification and control of contamination risk. These tools can be used to guide the design of rational biosafety strategies and inform the development of new technologies for bioreactor protection.
Life Science Research
Finding the miRNAs that Matter with Microcapillary Flow Cytometry
Topic: How the guava easyCyte™ flow cytometers enable miRNA profiling, and using the new SmartRNAplex™
Presenters: Sukhinder Sandhu, Ph.D., Swift BioSciences; and Daniel C. Pregibon, Ph.D., FireFly BioWorks
To elucidate the biological roles of microRNAs (miRNAs) and validate their utility as biomarkers, robust methods are needed to profile large sample cohorts. In this webinar, you will learn how guava easyCyte™ microcapillary flow cytometers enable miRNA profiling with exceptional throughput and high signal-to-noise ratios. Specifically, the guava easyCyte™ flow cytometers enable the new SmartRNAplex™ miRNA profiling assays, which can measure up to 68 targets simultaneously in a single well. The presentation, followed by live Q&A, features Dr. Sukhinder Sandhu (Ohio State University), who will describe her studies of tumor heterogeneity caused by overexpression of the miR-17-92 cluster. Dr. Daniel Pregibon (Firefly BioWorks) will then demonstrate how SmartRNAplex™ miRNA profiling assays are integrated into simple workflows on guava easyCyte™ flow cytometers.
During this webinar you'll learn:
How guava easyCyte™ microcapillary flow cytometers enable miRNA profiling.
The use of miRNAs as biomarker in tumors.
Using the new SmartRNAplex™ miRNA profiling assays for increased productivity.
The Message in the Haystack: Screening Live Stem Cells Using RNA Detection Probes
Topic: New tools for studying gene expression in live pluripotent stem cells
Presenters: Harald Lahm, Head of Laboratory of Molecular and Cell Biology of Experimental Surgery, German Heart Center Munich; and Don Weldon, Lead Scientist, Application Development, EMD Millipore
This online seminar will provide an overview of new tools for screening stem cells — in particular, the use of RNA detection probes to detect pluripotency gene expression in live embryonic and induced pluripotent stem cells by fluorescence microscopy without the need for manipulation of the cells.
Since their introduction in 2012, EMD Millipore's SmartFlare™ RNA detection probes have been cited in numerous publications, for studying gene expression in individual, live cells. This technique is widely applicable in dissecting heterogeneous cell populations across multiple cell types and research areas, including oncology, immunology, and cardiovascular research.
In this webinar, Don Weldon (Lead Scientist, EMD Millipore) will first provide an overview of the SmartFlare™ technology and its ability to study the gene expression of population subsets. Next, Dr. Harald Lahm (Head of the Laboratory of Experimental Surgery, German Heart Center Munich) describes the detection of various pluripotency genes in live, murine embryonic stem cells using SmartFlare probes. The same probes were also successfully applied to iPS cells of murine, human and porcine origin.
You will also learn about Dr. Lahm’s use of SmartFlare™ probes as a live screening tool to identify truly reprogrammed murine iPS cells derived from murine tail-tip fibroblasts in situ, based on their fluorescence intensity.