Proprietary Technologies Available for Licensing
293-Free™ Transfection Reagent features a proprietary polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is derived from non-animal sources and is ideal for mammalian protein production. This reagent is sold for research use and requires a license from EMD Millipore for any commercial use.
The AccepTor™ Vector Cloning Kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (i.e. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor Vectors enable direct ligation by virtue of single 3′-dU overhangs that anneal efficiently with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation. Protected under US Patent 5,856,144.
The Amplifluor® technology is a novel amplification and detection methodology that uses fluorescence energy transfer labels to perform quantitative PCR in a closed-tube format. This system consists of energy transfer labeled hairpin primers that produce a fluorescent signal only through incorporation into the amplification product. This technology allows for real-time monitoring of PCR as the fluorescence emitted is proportional to the amount of specific amplicon produced. Endpoint analysis can also be performed using this method. The signal can be detected by a variety of devices including fluorescent microtiter plate readers, spectrofluorometers, fluorescent imaging systems, and real-time instruments. Protected under US Patents 5,866,336, 6,090,552, 6,117,635, and related foreign equivalents.
BRCA1 and BRCA2 are important gene targets in the investigation and diagnosis of breast and ovarian cancers. The BRCA1 and BRCA2 genes including their polypeptides and antibodies are protected under US Patents 5,747,282, 5,753,441, and 6,162,897 (BRCA1) and US Patents 5,837,492 and 6,124,104 (BRCA2), which have been exclusively licensed in the research field by Myriad Genetics, Inc. to EMD Millipore.
GAB1, also known as GRB2-associated binding protein 1 is a member of the IRS1 (insulin receptor substrate 1)-like multisubstrate docking protein family. It recruits phosphatidylinositol-3 kinase (PI-3 kinase) and other effector proteins in response to the activation of several RTKs (receptor tyrosine kinases). Upon stimulation of RTKs, GAB1 is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold, mediating branching tubulogenesis and playing a central role in cellular growth response, transformation, and apoptosis. Protected under US Patent 6,133,428.
The Glutathione S-transferase (GST) gene fusion system is an integrated system for the expression, purification, and detection of fusion proteins. Protected under US Patent 5,654,176 and CA Patent 1,338,903. Commercial applications including but not limited to: (i) generation of antibodies against GST fusion proteins; (ii) production of GST fusion proteins for resale; (iii) research using the GST expression system that results in a commercial product, all require a license to these patents. GE Healthcare has exclusive rights (including sublicensing rights) for sale of expression vectors in the research market.
Integrin-linked kinase (ILK) is a serine-threonine kinase that is implicated in cell-to-extracellular matrix interaction, growth factor signaling, cell survival, cell migration, invasion, anchorage-independent growth, and angiogenesis. Increased expression of ILK has been associated with the progression of several tumor types. The ILK nucleic acid sequence, polypeptide, and antibodies are protected under US Patents 6,001,622, 6,013,782, 6,338,958, 6,369,205, 6,699,983, 7,189,802, and related foreign equivalents.
JAK kinases are a series of proteins involved in the regulation of the immune system and cell proliferation. Through the phosphorylation of STATs, these kinases play a critical role in the cytokine and growth factor signal transduction pathways. The JAK1 and JAK2 nucleic acid sequences, polypeptides, and antibodies are protected under US Patents 5,658,791, 5,716,818, 5,821,069, 5,852,184, 5,910,426, and related foreign equivalents.
Mitogen-activated protein (MAP) kinase kinase (MAP2K or MKK) mediates unique signal transduction pathways that activate MAP kinases p38 and JNK, which result in downstream activation of activating transcription factor-2 (ATF2) and c-Jun. MAP kinase pathways are activated by a number of factors, including cytokines and environmental stress. Protected under US Patent 6,174,676 and related foreign equivalents.
Protein methylation is a post-translational modification that plays an important role in several cellular mechanisms, including transcriptional regulation, signal transduction, RNA metabolism, and DNA repair. N-methylation is performed by the PRMT (protein arginine N-methyltransferase) family of enzymes, which introduces methyl groups on arginine residues in proteins, thereby modulating their function. Methylarginine-specific antibodies are protected under US Patent 6,699,673.
Mitogen-and-Stress-Activated Protein Kinase 1 and 2 (MSK1, MSK2) are unique nuclear serine/threonine protein kinases containing two kinase domains within a single polypeptide. These kinases are believed to mediate signal transduction in the inflammatory response and regulate cellular proliferation and differentiation. MSK1 and MSK2 are activated by the MAPK/ERK pathway (mitogen/growth factor signaling) or SAPK2/p38 pathway (stress/oxidation signaling) and activate transcription factors ATF-1 CREB. Protected under US Patent 7,122,360 and related foreign equivalents.
The NusA protein is an E. coli protein that was identified by solubility modeling as having a very high probability of being soluble when expressed in E. coli. When fused to heterologous proteins, NusA has been shown to increase the solubility of target proteins in E. coli, thus circumventing the formation of inclusion bodies common to heterologous protein expression in E. coli. Nus-Tag® Vectors have been developed by EMD Millipore to allow cloning and high-level expression of the NusA protein fused to a protein of interest. Protected under US Patents 5,989,868 and 6,207,420.
The OrientExpress™ cDNA Synthesis and Cloning Systems are complete sets of reagents designed for rapid, efficient construction of cDNA libraries having inserts in a defined orientation. The OrientExpress Random Primer System is based on the formation of directionally clonable randomly primed cDNA molecules. These cDNA molecules are then ligated directionally into a vector and subsequently prepared as a cDNA library, thus generating a population of randomly primed directional cDNA clones. Protected under US Patent 5,629,179. Overnight Express™ Autoinduction System The Overnight Express™ Autoinduction System is a method for promoting auto-induction of transcription of cloned DNA in cultures of bacterial cells, such transcription being under control of a promoter dependent on the metabolic state of the bacterial cells. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique auto-induction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems. Protected under US Patents 7,560,264, 7,704,722, and 7,759,109.
Cyclin-dependent kinase (CDK) inhibitors p14 and p15 act as tumor suppressor genes and are frequently deleted or mutated in a wide variety of human tumors. Protected under US Patents 5,739,027 and 6,060,301 (p14) and US Patents 5,994,095, 6,140,473, 6,180,776, 6,210,949, and 6,218,146 (p15), which have been exclusively licensed in the research field by Myriad Genetics, Inc. to EMD Millipore.
Pellet Paint® Co-Precipitant is a visible dye-labeled glycogen carrier formulated specifically for use in alcohol precipitation of nucleic acids. The two-minute precipitation uses microliter amounts per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions and the pellet is easily located and followed during washing steps by its vivid pink color. Protected under US Patents 7,144,713, 7,550,447, and related foreign equivalents.
The pET System allows for cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET vectors under control of the T7 promoter. Target gene expression is then induced by providing a source of T7 RNA Polymerase in the host cell. Protected under US Patent 5,693,489 licensed from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of the pET System. In addition, commercial use of the pET System requires a commercial license from EMD Millipore.
PopCulture Reagent is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification can be performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix:target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Protected under EU Patent 1,432,822.
PTEN is a tumor suppressor protein located on human chromosome 10 that is often mutated in various types of advanced cancers. The PTEN protein exhibits protein phosphatase activity and can suppress the growth of glioma cells. The PTEN gene including its polypeptide and antibodies are protected under US Patents 6,262,242, 6,482,795, and 7,217,795, which have been exclusively licensed in the research field by Myriad Genetics, Inc. to EMD Millipore.
The pTriEx™ Expression Vectors are triple host vectors capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vectors include three promoters, β-actin or CMV (vertebrate), T7lac (bacterial), and p10 (insect), such that the protein coding sequence can be cloned into the vector once and then allow for protein expression in all three types of host cells. The arrangement and selection of the promoters contribute to expression characteristics in all three host systems that are comparable to single host expression vectors. Protected under US Patent 6,589,783.
ReNcell VM and ReNcell CX are two well-established neural stem cell lines derived from developing human brains. ReNcell VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. These cells are sold for a six-month evaluation period and require a commercial license from EMD Millipore for any use beyond this initial evaluation period.
Serum and glucocorticoid-induced protein kinase (SGK) is a class of serine/threonine protein kinases induced following cellular exposure to serum or glucocorticoids. SGK is also induced in response to other stimuli, such as follicle stimulating hormone (FSH), induced extracellular osmolarity, and transfection of mammary epithelial cells with p53. SGK is activated by IGF-1 or oxidative stress via the phosphatidylinositol (PI)-3-kinase pathway. Once activated, SGK preferentially inactivates GSK3 via phosphorylation. Protected under US Patent 7,083,511.
The suppressors of cytokine signaling (SOCS) are a family of intracellular protein that regulate cytokine responses. The SOCS proteins regulate signal transduction by combining direct inhibitory interactions with cytokine receptors and signaling proteins. Protected under US Patent 6,323,317.
The T7Select® system is a protein display system based on bacteriophage T7 that features a number of unique attributes. Phage assembly takes place inside the E. coli cell and mature phage are released by cell lysis. In contrast to filamentous phage assembly, peptides or proteins displayed on the surface of the T7 phage do not need to be capable of secretion through the cell membrane. Protected under US Patent 5,766,905.