Cellulose HPTLC Plates


High-Performance Separations of Polar Compounds

Cellulose HPTLC plates for analysis of polar compoundsCellulose is an organic sorbent, which is particularly suitable for the separation of hydrophilic substances by partition chromatography. MilliporeSigma’s high-quality cellulose plates include classical TLC or HPTLC grades, and facilitate a wide range of separations. Both plates are available with or without a special fluorescent indicator, which is stimulated to intense blue fluorescent emission at long-wave UV light of 366 nm, and at short-wave UV light of 254 nm.

TLC cellulose layers: Based on microcrystalline cellulose for standard separations

HPTLC cellulose layers: Based on high-purity, rod-shaped microcrystalline cellulose, resulting in a highly reduced diffusion of analytes for critical high-performance separations

Features and Benefits

  • Ideal for partition chromatography of hydrophilic substances
  • High-performance separations due to greatly reduced diffusion of analytes
  • Easier detection through intense fluorescent indicator

Application Areas

Typical applications of cellulose plates include the analysis of amino acids, carbohydrates, phosphates, nucleic acids and nucleic acid derivatives for:

  • Two-dimensional separations, such as amino acid “fingerprints”
  • Metabolic studies

Separation of Phosphates


Compounds 1. (NaPO3)3
2. Na5P3O10
3. Na4P2O7
4. Na2HPO4 all 0.2%
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-TLC and HPTLC-TLC HPTLC cellulose-480x552-03172014.jpg
Sample volume  250 nL
Mobile phase 16% trichloroacetic acid diocane sol. ammonia in 1 L water; 70/30
Migration distance  7 cm
Detection  586 nm, TLC/HPTLC scanner, Camag
 
 
 
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