Classic particulate columns versus Chromolith® HPLC columns
The use of HPLC columns containing the classic 3 or 5 μm small silica particles often results in high back pressure. This may damage both the column and the HPLC system; therefore, classic HPLC columns have limited length and a limited number of theoretical plates. Attempts have been made to increase the plate count by decreasing the particle size, but this results in unacceptable back pressure and limits the variety of separations that can be satisfactorily achieved.
The development of faster separation processes has become one of the most important issues in high-performance liquid chromatography. Modern laboratories utilize automation of HPLC systems which feature around the clock operations . To accelerate the process even more, there’s no better choice than Chromolith® HPLC columns. Thanks to their revolutionary bimodal pore structure, Chromolith® columns provide excellent separations in a fraction of the time that a standard particulate column takes. This enables higher speeds and higher sample throughput at lower back pressure.