A part of MilliporeSigma

17-470 | phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit

96 assays  
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      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Met H ELISA Chromogenic, Colorimetric
      Catalogue Number17-470
      Brand Family Upstate
      Trade Name
      • STAR
      • Upstate
      Descriptionphospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit
      Background InformationTest Principle:
      The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Met plate is coated with a specific mouse monoclonal anti-Met capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing Met antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) antibody to detect the captured phospho-Met (Tyr1230/Tyr1234/Tyr1235) on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.

      Met Background:
      Met is a tyrosine kinase receptor for Hepatocyte Growth Factor (HGF), thought to stimulate multiple cellular processes including cell proliferation, differentiation, cell migration and tumorigenesis. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis.
      Materials Required but Not Delivered1. Multi-channel or repeating pipettes
      2. Plate shaker (optional)
      3. Pipettors & tips capable of accurately measuring 1-1000µL
      4. Graduated serological pipettes
      5. 96-well microtiter Plate Reader with 450 nm filter
      6. Graphing software for plotting data or graph paper for manual plotting of data
      7. Microfuge tubes for standard and sample dilutions
      8. Mechanical vortex
      9. 1 liter container
      10. Distilled or deionized water
      Product Information
      • 1. Met Capture Plate: (Part No. 17-470A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
      • 2. Anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) detection antibody: (Part No. 17-470B) One bottle (11 mL) of anti-phospho-Met (Tyr1230/Tyr1234/Tyr1235) detection antibody containing sodium azide, ready to use.
      • 3. ELISA Diluent: (Part No. 17-470C) One bottle (25 mL) of ELISA Diluent sodium azide, ready to use.
      • 4. 25X ELISA Wash Buffer: (Part No. 17-470D) One bottle (50 mL) of 25X ELISA Wash Buffer.
      • 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-470E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.
      • 6. HRP Diluent: (Part No. 17-470F) One bottle (25 mL) of HRP diluent containing thimerosol.
      • 7. TMB Solution: (Part No. 17-470G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.
      • 8. Stop Solution: (Part No. 17-470H) One bottle (25 mL) of stop solution, ready to use.
      • 9. phospho-Met (Tyr1230/Tyr1234/Tyr1235) Standard: (Part No. 17-470I) Two vials of phospho-Met (Tyr1230/Tyr1234/Tyr1235) standard, lyophilized.
      • 10. Plate Covers: Two plate covers.
      Detection methodChromogenic Colorimetric
      ApplicationThe phospho-Met (Tyr1230/Tyr1234/Tyr1235) colorimetric STAR ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to quantify specific levels of signaling targets in denatured cell extracts.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Met
      Entrez Gene Number
      Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
      Gene Symbol
      • MET
      • HGFR
      • AUTS9
      • c-Met
      • RCCP2
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
      SIZE: 1390 amino acids; 155527 Da
      SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
      Physicochemical Information
      • Sensitivity: 0.78 Units/mL.
        Range of Detection: 1.6 to 50 Units/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by ELISA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C
      Packaging Information
      Material Size96 assays
      Transport Information
      Supplemental Information

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