70174 pT7Blue Blunt Vector (linearized) - Novagen

70174
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      Overview

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      70174-3
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          Plastic ampoule 20 rxn
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              Plastic ampoule 40 rxn
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              Description
              Overview

              The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.



              Catalogue Number70174
              Brand Family Novagen®
              Features and benefits
              • No restriction enzymes or special primers
              • Compatible with any DNA polymerase
              • Independent of 3ʹ-dA addition or sequence context of DNA ends
              • Blue/white screening with seven different vectors, including pETBlue expression vectors
              • Simple protocol takes less than one hour* from PCR product to plating transformants

              * All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.

              References
              References

              Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

              Product Information
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              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Standard Handling
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
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              Supplemental Information
              Specifications

              Documentation

              pT7Blue Blunt Vector (linearized) - Novagen SDS

              Title

              Safety Data Sheet (SDS) 

              pT7Blue Blunt Vector (linearized) - Novagen Certificates of Analysis

              TitleLot Number
              70174

              References

              Reference overview

              Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

              Citations

              Title
            • Ekaterina Bogdanova, et al. (2008) Transcription regulation of the type II restriction-modification system AhdI. Nucleic Acids Research 36, 1429-1442.
            • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
            • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
            • Rahul Pal, et al. (2005) Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses. Journal of Immunology 175, 7669-7677.
            • Dana R. Rundle, et al. (2004) Myristoyl-CoA: protein N-myristoyltransferases: isoform identification and gene expression in retina. Molecular Vision 10, 177-185.
            • User Protocols

              Title
              TB183 Perfectly Blunt® Cloning Kits

              Vector Map

              Title
              TB017VM pT7Blue Vector Map

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              Categories

              Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors