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71363 | pRSF-1b DNA - Novagen

71363
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      71363-3
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          Glass bottle 10 μg
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          Description
          OverviewThe pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag® coding sequence that results in native protein after purification and cleavage. This plasmid carries an origin derived from RSF1030 (1, 2) and kanamycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors. Please read technical bulletin, TB401, for all possibilities and contact technical service if you need additional information.

          This plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional S•Tag™ coding sequence is present at the distal end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (3).
          Catalogue Number71363
          Brand Family Novagen®
          References
          References1. Som, T. and Tomizawa, J. (1982) Mol. Gen. Genet. 187, 375–383.

          2. Cannon, P.M. and Strike, P. (1992) Plasmid 27, 220–230.

          3. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
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          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          71363

          References

          Reference overview
          1. Som, T. and Tomizawa, J. (1982) Mol. Gen. Genet. 187, 375–383.

          2. Cannon, P.M. and Strike, P. (1992) Plasmid 27, 220–230.

          3. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

          Citations

          Title
        • Jingjing Xie, et al. (2007) Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches. Journal of Biological Chemistry 282, 4218-4231.
        • Olivier Berteau, et al. (2006) A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes. Journal of Biological Chemistry 281, 22464-22470.
        • User Protocols

          Title
          TB401 pRSF and pCDF Vectors

          Vector Map

          Title
          TB393VM pRSF-1b Vector Map

          Vector Sequence

          Title
          pRSF-1b Vector Sequence