69659 pLysS DNA - Novagen

69659
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      Overview

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      69659-3
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          Glass bottle 1 μg
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          Description
          OverviewpLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

          This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.





          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number69659
          Brand Family Novagen®
          References
          References1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          pLysS DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pLysS DNA - Novagen Certificates of Analysis

          TitleLot Number
          69659

          References

          Reference overview
          1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.

          Citations

          Title
        • Pernilla Larsson Birgander, et al. (2005) Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1. Journal of Biological Chemistry 280, 14997-15003.
        • Dale K. Shumaker, et al. (2005) Functions and dysfunctions of the nuclear lamin Ig-fold domain in nuclear assembly, growth, and Emery-Dreifuss muscular dystrophy. Proceedings of the National Academy of Sciences (USA) 102, 15494-15499.
        • Vector Map

          Title
          TB107 pLysS and pLysE Plasmid Map

          Vector Sequence

          Title
          pLysS Sequence

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors