Pricing & Availability
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|71241-3||Glass bottle||20 μg||
|Overview||The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-1 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
For sequencing or PCR of recombinants in pIEx-1, refer to the table below to choose the appropriate primers.
|Synonyms||InsectDirect™ System- pIEx™-1 DNA|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Shipped with Blue Ice or with Dry Ice|
|Do not freeze||Ok to freeze|
|Insect Cell Expression|
|Insect Direct!" Protein Expression & Purification System|
|TB356 InsectDirect™ pIEx™ Vectors|
|TB349VM pIEx™-1 Vector Map|
|pIEx™-1 Vector Sequence|