70557 | pET-41b(+) DNA - Novagen

70557
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      Overview

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      70557-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-41a-c(+) and pET-42a-c(+) vectors incorporate the schistosomal glutathione-S-transferase (GST; GST•Tag) coding sequence as a fusion partner. The GST•Tag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners (Smith 1998). When expressed in a soluble, properly folded form, GST•Tag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. The pET-41 and -42 series feature the powerful T7lac promoter, and encode the GST•Tag (220 aa) sequence, proteolytic sites, His•Tag (6 aa) sequence, and S•Tag (15 aa) sequence.

          In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow removal of 100% of the vector-encoded sequences and the generation of native proteins with their authentic N-terminal residues. Unfused proteins can be produced by using the Nde I cloning site. A version of pET-41 is available as a linearized vector prepared for ligation-independent cloning (LIC) of PCR products.

          Another pET vector with the GST•Tag sequence is pET-49b(+). Please see the website description for more information. The His•Tag and S•Tag sequences enable alternative protein detection and purification procedures to be performed. For example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.



          The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB239). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number70557
          Brand Family Novagen®
          References
          References

          Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.

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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          70557

          References

          Reference overview

          Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

          Citations

          Title
        • Johnna L. Roose, Yasuhiro Kashino and Himadri B. Pakrasi. (2007) The PsbQ protein defines cyanobacterial photosystem II complexes with highest activity and stability. Procedings of the National Academy of Science 104, 2548-2553.
        • Keiko Yonekura-Sakakibara, et al. (2007) Identification of a flavonol 7-O-rhamnosyltransferase gene determining flavonoid pattern in Arabidopsis by transcriptome coexpression analysis and reverse genetics. Journal of Biological Chemistry 282, 14932-14941.
        • Hendrik Adams, et al. (2006) Interaction of tonB with the outer membrane receptor FpvA of Pseudomonas aeruginosa. Journal of Bacteriology 188, 5752-5761.
        • Eric A. Schmelz, et al. (2006) Fragments of ATP synthase mediate plant perception of insect attack. Proceedings of the National Academy of Sciences (USA) 103, 8894-8899.
        • Victor J. Torres, et al. (2005) Functional properties of the p33 and p55 domains of the Helicobacter pylori vacuolating cytotoxin. Journal of Biological Chemistry 280, 21107-21114.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB239VM pET-41a-c(+) Vector Map

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          70556 pET-41a(+) DNA - Novagen Show Pricing & Availability
          70561 pET-42a(+) DNA - Novagen Show Pricing & Availability
          70562 pET-42b(+) DNA - Novagen Show Pricing & Availability

          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors