69017 pET-32c(+) DNA - Novagen

69017
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69017-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe pET-32c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB122VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69017
          Brand Family Novagen®
          References
          References1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.
          Product Information
          Vector familypET
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-32c(+) DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-32c(+) DNA - Novagen Certificates of Analysis

          TitleLot Number
          69017

          References

          Reference overview
          1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)

          Citations

          Title
        • Hann Ling Wong, et al. (2007) Regulation of rice NADPH oxidase by binding of rac GTPase to its N-terminal extension. Plant Cell 19, 4022-4034.
        • Kristel Vercauteren, et al. (2006) PGC-1-related coactivator: immediate early expression and characterization of a CREB/NRF-1 binding domain associated with cytochrome c promoter occupancy and respiratory growth. Molecular and Cellular Biology 26, 7409-7419.
        • King-Tung Chin, et al. (2005) The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma. Nucleic Acids Research 33, 1859-1873.
        • Anna B. Gilg, et al. (2005) Isolation and functional expression of an animal geranyl diphosphate synthase and its role in bark beetle pheromone biosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 9760-9765.
        • Chungho Kim, et al. (2005) Filamin is essential for shedding of the transmembrane serine protease, epithin. European Molecular Biology Organization Reports 6, 1045-1051.
        • Han Ie Kim and Seung-Taek Lee. (2005) An intramolecular interaction between SH2-kinase linker and kinase domain is essential for the catalytic activity of protein tyrosine kinase-6. Journal of Biological Chemistry 280, 28973-28980.
        • Philip J. Young, et al. (2003) The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein. Molecular Brain Research 119, 37-49.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB122VM pET-32a-c(+) Vector Map

          Vector Sequence

          Title
          pET-32c(+) Vector Sequence

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors