69864 pET-28a(+) DNA - Novagen

69864
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      69864-3
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          Glass bottle 10 μg
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          Description
          OverviewThe pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins.
          The pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB074).

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69864
          Brand Family Novagen®
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          Storage ≤ -70°C
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          Do not freeze Ok to freeze
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          pET-28a(+) DNA - Novagen SDS

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          Safety Data Sheet (SDS) 

          pET-28a(+) DNA - Novagen Certificates of Analysis

          TitleLot Number
          69864

          Citations

          Title
        • Mintu Desai and Jianping Hu. (2008) Light induces peroxisome proliferation in Arabidopsis seedlings through the photoreceptor phytochrome A, the transcription factor HY5 homolog, and the peroxisomal protein peroxin11b. Plant Physiology 146, 1117-1127.
        • Valeria Genovesi, et al. (2008) ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana. Journal of Experimental Botany 59, 875-889.
        • Mateen A. Khan, et al. (2008) Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and EIFiso4F. Journal of Biological Chemistry 283, 1340-1349.
        • Bidyottam Mittra, et al. (2008) The TbMTr1 spliced leader RNA cap 1 2 '-O-Ribose methyltransferase from Trypanosoma brucei acts with substrate specificity. Journal of Biological Chemistry 283, 3161-3172.
        • Sunish Kumar Radhakrishnan, Martin Thanbichler and Patrick H. Viollier. (2008) The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Genes and Development 22, 212-225.
        • A. Reza Radjabi, et al. (2008) Thrombin induces tumor invasion through the induction and association of matrix metalloproteinase-9 and 1-integrin on the cell surface. Journal of Biological Chemistry 283, 2822-2834.
        • Dashuang Shi, et al. (2008) The crystal structure of N-acetyl-L-glutamate synthase from Neisseria gonorrhoeae provides insights into mechanisms of catalysis and regulation. Journal of Biological Chemistry 283, 7176-7184.
        • Shalini Thakran, et al. (2008) Identification of Francisella tularensis lipoproteins that stimulate the Toll-like receptor (TLR) 2/TLR1 heterodimer. Journal of Biological Chemistry 283, 3751-3760.
        • Nicholas E. Burgis and Richard P. Cunningham. (2007) Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase. Journal of Biological Chemistry 282, 3531-3538.
        • Vanessa Gonzalez-Covarrubias, et al. (2007) A functional genetic polymorphism on human carbonyl reductase 1 (CBR1) impacts on catalytic activity and NADPH binding affinity.. Drug Metabolism and Disposition 35, 973-980.
        • Bei Han, et al. (2007) Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41. Applied and Enviornmental Microbiology 73, 3581-3586.
        • Marcel Hohl, et al. (2007) Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity. Nucleic Acids Research 35, 3053-3063.
        • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
        • J. Kramer, et al. (2007) Citrate sensing by the C4-dicarboxylate/citrate sensor kinase DcuS of Escherichia coli: binding site and conversion of DcuS to a C4-dicarboxylate- or citrate-specific sensor. Journal of Bacteriology 189, 4290-4298.
        • Peter Naur, et al. (2007) Ionotropic glutamate-like receptor δ2 binds D-serine and glycine. Procedings of the National Academy of Science 104, 14116-14121.
        • Biagio Pucci, et al. (2007) Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein. Journal of Biological Chemistry 282, 12574-12582.
        • Yasuo Sakamoto, et al. (2007) Overlapping roles of the methylated DNA-binding protein MBD1 and polycomb group proteins in transcriptional repression of HOXA genes and heterochromatin foci formation. Journal of Biological Chemistry 282, 16391-16400.
        • Ferdinand Steinboeck, et al. (2007) The nuclear actin-related protein of Saccharomyces cerevisiae, Arp4, directly Interacts with the histone acetyltransferase Esa1p. Journal of Biochemistry (Tokyo) 141, 661-668.
        • Ken-ichiro Taoka, et al. (2007) Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement. Plant Cell 19, 1866-1884.
        • Susanne Textor, et al. (2007) MAM3 catalyzes the formation of all Aliphatic glucosinolate chain lengths in Arabidopsis. Plant Physiology 144, 60-71.
        • Daqi Tu, et al. (2007) Structure and function of the yeast U-box-containing ubiquitin ligase Ufd2p. Procedings of the National Academy of Science 104, 15599-15606.
        • Spencer M. Whitney and Robert E. Sharwood. (2007) Linked rubisco subunits can assemble into functional oligomers without impeding catalytic performance. Journal of Biological Chemistry 282, 3809-3818.
        • Jingjing Xie, et al. (2007) Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches. Journal of Biological Chemistry 282, 4218-4231.
        • Liguo Zhang, Kenneth P. Nephew and Patricia J. Gallagher. (2007) Regulation of death-associated protein kinase: stabilization by HSP90 heterocomplexes. Journal of Biological Chemistry 282, 11795-11804.
        • Luke J. Alderwick, et al. (2006) Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences (USA) 103, 2558-2563.
        • Olivier Berteau, et al. (2006) A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes. Journal of Biological Chemistry 281, 22464-22470.
        • Gina Boanca, Aaron Sand and Joseph J. Barycki. (2006) Uncoupling enzymatic and autoprocessing activities of H. pylori γ-glutamyltranspeptidase. Journal of Biological Chemistry 281, 19029-19037.
        • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
        • Frédéric Chédin, et al. (2006) The AddAB helicase/nuclease forms a stable complex with its cognate sequence during translocation. Journal of Biological Chemistry 281, 18610-18617.
        • Zahaed Evangelista-Martínez, Gabriela González-Cerón and Luis Servín-González. (2006) A Conserved Inverted Repeat, the LipR Box, Mediates Transcriptional Activation of the Streptomyces exfoliatus Lipase Gene by LipR, a Member of the STAND Class of P-Loop Nucleoside Triphosphatases. Journal of Bacteriology 188, 7082-7089.
        • Jeffrey S. Fillingham, et al. (2006) Molecular genetic analysis of an SNF2/brahma-related gene in Tetrahymena thermophila suggests roles in growth and nuclear development. Eukaryotic Cell 5, 1347-1359.
        • Li Ge and Stephen Y. K. Seah. (2006) Heterologous expression, purification, and characterization of an L-ornithine N5-hydroxylase involved in pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology 188, 7205-7210.
        • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
        • Jeff W. Hill and Michele K. Evans. (2006) Dimerization and opposite base-dependent catalytic impairment of polymorphic S326C OGG1 glycosylase. Nucleic Acids Research 34, 1620-1632.
        • Antonio A. Iniesta, et al. (2006) A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Procedings of the National Academy of Science 103, 10935-10940.
        • Ray S. Oh, Xinli Bai and Johanna M. Rommens. (2006) Human homologs of UbC6P ubiquitin-conjugating enzyme and phosphorylation of HSUbC6E in response to endoplasmic reticulum stress. Journal of Biological Chemistry 281, 21480-21490.
        • Sang-Youn Park, et al. (2006) Structure of FliM provides insight into assembly of the switch complex in the bacterial flagella motor. Procedings of the National Academy of Science 103, 11886-11891.
        • James F. Parsons, et al. (2006) Crystallization and X-ray diffraction analysis of salicylate synthase, a chorismate-utilizing enyme involved in siderophore biosynthesis. Acta Crystallographica F62, 271-274.
        • Debashish Ray, et al. (2006) West Nile virus 5'-cap structure is formed by sequential guanine N-7 and ribose 2'-O methylations by nonstructural protein 5. Journal of Virology 80, 8362-8370.
        • Stanislav V. Sosnovtsev, et al. (2006) Cleavage map and proteolytic processing of the murine norovirus nonstructural polyprotein in infected cells. Journal of Virology 80, 7816-7831.
        • Hong Ye, et al. (2006) CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation. Journal of Biological Chemistry 281, 8958-8969.
        • Chaitali Basak, et al. (2005) The secreted peptidyl prolyl cis,trans-isomerase HP0175 of Helicoba
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB074VM pET-28a-c(+) Vector Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors