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69744 | pET-22b(+) DNA - Novagen

69744
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69744-3
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          Glass bottle 10 μg
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          Description
          OverviewThe pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins.
          The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB038).

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number69744
          Brand Family Novagen®
          References
          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          69744

          Citations

          Title
        • Arima, J., et al. 2008. Protein Engineering Design and Selection 21, 45.
        • Kimberly A. Burkhard and Angela Wilks. 2007. Journal of Biological Chemistry 282, 15126.
        • Cao, et al. W., 2007. Journal of Biological Chemistry 282, 18922.
        • Fujii, T., et al. 2007. Journal of Biochemistry (Tokyo) 141, 699.
        • Mark S. McClain and Timothy L. Cover. 2007. Infection and Immunity 75, 1785.
        • Nishiwaki, H., et al. 2007. Applied and Enviornmental Microbiology 73, 3404.
        • Roy, L.M., et al. 2007. Journal of Biological Chemistry 282, 15790.
        • Katja Strube, Simon de Vries and Rainer Cramm. 2007. Journal of Biological Chemistry 282, 20292.
        • Elizebeth C. Turner and Charles J. Dorman. 2007. Journal of Bacteriology 189, 3403.
        • Williams, E.P., et al. 2007. Journal of Bacteriology 189, 4234.
        • Chédin, F., et al. 2006. Journal of Biological Chemistry 281, 18610.
        • Langenheim, J.F., et al. 2006. Molecular Endocrinology 20, 661.
        • Carmelo, E., et al. 2005. Journal of Biological Chemistry 280, 28683.
        • Csizmok, V., et al. 2005. Molecular and Cellular Proteomics 5, 265.
        • Elia, N., et al. 2005. Journal of Cell Biology 171, 517.
        • Fierobe, H-P., et al. 2005. Journal of Biological Chemistry 280, 16325.
        • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
        • Loiseau, L., et al. 2005. Journal of Biological Chemistry 280, 26760.
        • Lucet, I.S., et al. 2005. Journal of Biological Chemistry 280, 2503.
        • Moore, J., et al. 2005. Journal of Biological Chemistry 280, 31489.
        • Oh, K.J., et al. 2005. Journal of Biological Chemistry 280, 753.
        • Amit V. Pandey and Walter L. Miller. 2005. Journal of Biological Chemistry 280, 13265.
        • Proctor, M.R., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 2697.
        • Varrot, A., et al. 2005. Journal of Biological Chemistry 280, 20181.
        • Williams, J.C., et al. 2005. Journal of Biological Chemistry 15202.
        • Yatsula, B., et al. 2005. Journal of Biological Chemistry 280, 30712.
        • Zhou, H., et al. 2005. Journal of Biological Chemistry 280, 13841.
        • Zhu, G., et al. 2005. Journal of Biological Chemistry 280, 23057.
        • Dong, T., et al. 2004. Journal of Experimental Medicine 200, 1547.
        • Hiroyasu Furukawa and Eric Gouaux. 2003. EMBO J. 22, 2873.
        • Seybert, A., et al. 2002. Nucleic Acids Research 30, 4329.
        • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. 2001. Procedings of the National Academy of Science 98, 445.
        • Jae Hyung Koo, Ick Hyun Cho and Yu Sam Kim. 2000. Journal of Bacteriology 182, 6382.
        • Z. Lin, T. Thorsen and F.H. Arnold. 1999. Biotechnol. Prog. 15, 467.
        • Gavin MacBeath, Peter Kast and Donald Hilvert. 1998. Science 279, 1958.
        • MacKenzie-Graham, A.J., et al. 1997. Journal of Immunology 159, 4601.
        • Ralph H. Lambalot and Christopher T. Walsh. 1995. Journal of Biological Chemistry 270, 24658.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB038VM pET-22b(+) Vector Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors