71406 pCOLADuet™-1 DNA - Novagen

71406
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71406-3
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          Glass bottle 10 μg
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          Description
          OverviewpCOLADuet™-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag® sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to eight target genes.






          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71406
          Brand Family Novagen®
          References
          References1. Zverev, V. V. and Khmel, I. A. (1985) Plasmid 14, 192–199.
          Product Information
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          Safety Information according to GHS
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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
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          Documentation

          pCOLADuet™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pCOLADuet™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          71406

          References

          Reference overview
          1. Zverev, V. V. and Khmel, I. A. (1985) Plasmid 14, 192–199.

          Citations

          Title
        • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
        • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
        • User Protocols

          Title
          TB340 Duet Vectors
          TB408 pCOLADuet™-1 Vector Map

          Vector Sequence

          Title
          pCOLADuet-1 Vector Sequence