70003 pBAC™-1 DNA - Novagen

70003
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70003-3
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          Plastic ampoule 10 μg
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          Description
          OverviewpBAC™-1 is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. Inserts in pBAC-1 must contain their own translation initiation codon. The vector encodes an optional C-terminal His•Tag® fusion sequence that may be utilized if the insert allows read-through in the proper reading frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70003
          Brand Family Novagen®
          References
          Product Information
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          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pBAC™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pBAC™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          70003

          Citations

          Title
        • Zhongmin Ma, et al. (1999) Human pancreatic islets express RNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on the chromosome. Journal of Biological Chemistry 274, 9607-9616.
        • Antonio Alcami, et al. (1998) Blockade of chemokine activity by a soluble chemokine binding protein from vaccinia virus. Journal of Immunology 160, 624-633.
        • Robert Bals, et al. (1998) Human defensin 2 Is a salt-sensitive peptide antibiotic expressed in human lung. Journal of Clinical Investigation 102, 874-880.
        • Vector Map

          Title
          TB127 pBAC-1 Vector Map

          Vector Sequence

          Title
          pBAC-1 Sequence

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits