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14-784 | mTOR Protein and FKBP12 Protein, 1 kit

1 kit  
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      Replacement Information
      Catalogue Number14-784
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionmTOR Protein and FKBP12 Protein, 1 kit
      OverviewThis product includes 10 µg of active mTOR (1362-end) and 1 mg of FKBP12.
      Background InformationFKBP12: N-terminal 6His-tagged recombinant, full length, human FKBP12, expressed in E.coli.

      mTOR (1362-end), active: N-terminal FLAG™-tagged, recombinant, human mTOR, amino acids 1362-end, expressed by baculovirus in Sf21 insect cells.
      Product Information
      • mTOR (1362-end), active (Cat.# 14-770)
      • FKBP12
      PresentationmTOR Enzyme in 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25 mM sodium orthovanadate, 10 mM NaF, 300 μg/ml FLAG™ peptide, 20% v/v glycerol.

      FKBP12in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      ApplicationThis product includes 10 µg of active mTOR & 1 mg of FKBP12 (mTOR: N-terminal FLAG-tagged, recombinant, human, amino acids 1362-end. FKBP12: N-terminal 6His-tagged recombinant, human, full length).
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
      Gene Symbol
      • FRAP1
      • RAPT1
      • MTOR
      • FRAP
      • mTOR
      • FLJ44809
      • FRAP2
      • RAFT1
      Protein TargetmTOR (FRAP1)
      Target Sub-FamilyLipid/Atypical
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
      SIZE: 2549 amino acids; 288892 Da
      SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
      TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
      SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore -70°C
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information


      Related Products & Applications

      Kit Component

      Catalog Number Description  
      14-770 mTOR (1362-end) Protein, active, 10 µg Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases