420220 | iPSC Induction Enhancer, Thiazovivin - Calbiochem

420220
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₅H₁₃N₅OS

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      420220-10MG
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          Plastic ampoule 10 mg
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          Description
          OverviewA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461) and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.
          Catalogue Number420220
          Brand Family Calbiochem®
          SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
          References
          ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
          Lin, T., et al. 2009. Nat. Methods 6, 805.
          Product Information
          FormTan powder
          Hill FormulaC₁₅H₁₃N₅OS
          Chemical formulaC₁₅H₁₃N₅OS
          Structure formula ImageStructure formula Image
          Applications
          ApplicationiPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.
          Biological Information
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          420220

          References

          Reference overview
          Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
          Lin, T., et al. 2009. Nat. Methods 6, 805.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-April-2012 JSW
          SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
          DescriptionA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461) and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.
          FormTan powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₅H₁₃N₅OS
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (100 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
          Lin, T., et al. 2009. Nat. Methods 6, 805.