ZipTip® Pipette Tips

Concentrating and purifying samples for MALDI-ToF MS

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Overview

Specifications

Ordering Information

ZipTip® Pipette Tips Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
ZTC04S008ZipTip with 0.6 µL C4 resin 8 Show Pricing & Availability
ZTC04S096ZipTip with 0.6 µL C4 resin 96 Show Pricing & Availability
ZTC04S960ZipTip with 0.6 µL C4 resin 960 Show Pricing & Availability
ZTC18M008ZipTip with 0.2 µL C18 resin 8 Show Pricing & Availability
ZTC18M096ZipTip with 0.2 µL C18 resin 96 Show Pricing & Availability
ZTC18M960ZipTip with 0.2 µL C18 resin 960 Show Pricing & Availability
ZTC18S008ZipTip with 0.6 µL C18 resin 8 Show Pricing & Availability
ZTC18S096ZipTip with 0.6 µL C18 resin 96 Show Pricing & Availability
ZTC18S960ZipTip with 0.6 µL C18 resin 960 Show Pricing & Availability
ZTSCXS008ZipTip with 0.6 µL strong cation resin 8 Show Pricing & Availability
ZTSCXS096ZipTip with 0.6 µL strong cation resin 96 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    Purification of Radiolabeled Oligonucleotides Using ZipTipC18 Pipette Tips

    Data Sheet

    Title
    Mass Spectrometry Workflow Solutions

    Posters

    Title
    Enhanced MALDI-TOF MS Spectra of Serum Peptides Using Ultrafiltration ; ASMS 2002
    Poster: Purification of Peptides from Serum and Cell Lysates for Mass Spectrometry, ASMS, 2004
    Zip....Tips: Creative Ways to Use ZipTip Pipette Tips (ABRF 2003)
    ZipTip Chemistries Poster: Micro-Adsorptive Sample Preparation for Mass Spectrometry

    References

    Reference overviewApplicationPub Med ID
    A CROSS-SPECIES STUDY OF PI3K PROTEIN-PROTEIN INTERACTIONS REVEALS THE DIRECT INTERACTION OF P85 AND SHP2
    Susanne B. Breitkopf, Xuemei Yang, Michael J. Begley, Meghana Kulkarni, Yu-Hsin Chiu, Alexa B. Turke, Jessica Lauriol, Min Yuan, Jie Qi, Jeffrey A. Engelman, Pengyu Hong, Maria I. Kontaridis, Lewis C. Cantley, Norbert Perrimon, John M. Asara
    Sci Rep.  2016

    Show Abstract
    26839216 26839216
    FOOD PEPTIDOMICS OF IN VITRO GASTROINTESTINAL DIGESTIONS OF PARTIALLY PURIFIED BOVINE HEMOGLOBIN: LOW-RESOLUTION VERSUS HIGH-RESOLUTION LC-MS/MS ANALYSES
    Juliette Caron, Gabrielle Chataigne, Jean-Pascal Gimeno, Nathalie Duhal, Jean-Francois Goossens, Pascal Dhulster, Benoit Cudennec, Rozenn Ravallec, Christophe Flahaut
    Electrophoresis  2016

    Show Abstract
    26990205 26990205
    Comparative evaluation of peptide desalting methods for salivary proteome analysis.
    Nico Jehmlich, Claas Golatowski, Annette Murr, Gesell Salazar, Vishnu Mukund Dhople, Elke Hammer, Uwe Volker
    Clin Chim Acta  2014

    24751662 24751662
    ISOLATION AND IDENTIFICATION OF MEMBRANE VESICLE-ASSOCIATED PROTEINS IN GRAM-POSITIVE BACTERIA AND MYCOBACTERIA
    Rafael Prados-Rosales, Lisa Brown, Arturo Casadevall, Sandra Montalvo-Quiros, Jose L. Luque-Garcia
    MethodsX.  2014

    Show Abstract
    26150943 26150943
    Using theoretical protein isotopic distributions to parse small-mass-difference post-translational modifications via mass spectrometry.
    Timothy W. Rhoads, Jared R. Williams, Nathan I. Lopez, Jeffrey T. Morre, C. Samuel Bradford, Joseph S. Beckman
    J Am Soc Mass Spectrom  2013

    23247967 23247967
    DETERMINIG IN VIVO PHOSPHORYLATION SITES USING MASS SPECTROMETRY
    Susanne B. Breitkopf, John M. Asara
    Curr Protoc Mol Biol  2012

    Show Abstract
    22470061 22470061
    Rapid metabolite identification with sub parts-per-million mass accuracy from biological matrices by direct infusion nanoelectrospray ionization after clean-up on a ZipTip and LTQ/Orbitrap mass spectrometry.
    John C. L. Erve, William DeMaio, Rasmy E. Talaat
    Rapid Commun Mass Spectrom  2008

    Show Abstract
    18763271 18763271
    COMPARISON OF SDS- AND METHANOL-ASSISTED PROTEIN SOLUBILIZATION AND DIGESTION METHODS FOR ESCHERICHIA COLI MEMBRANE PROTEOME ANALYSIS BY 2-D LC-MS/MS
    Nan Zhang, Rui Chen, Nelson Young, David Wishart, Philip Winter, Joel H. Weiner, Liang Li
    Proteomics  2007

    17309111 17309111
    Analysis of modified apolipoprotein B-100 structures formed in oxidized low-density lipoprotein using LC-MS/MS.
    Takashi Obama, Rina Kato, Yutaka Masuda, Katsuhiko Takahashi, Toshihiro Aiuchi, Hiroyuki Itabe
    Proteomics  2007

    Show Abstract
    17549798 17549798
    GEL-FREE SAMPLE PREPARATION FOR THE NANOSCALE LC-MS/MS ANALYSIS AND IDENTIFICATION OF LOW-NANOGRAM PROTEIN SAMPLES
    Marco Gaspari, Vittorio Abbonante, Giovanni Cuda
    J Sep Sci.  2007

    Show Abstract
    17763504 17763504

    FAQ

    QuestionAnswer
    Are ZipTip pipette tips compatible with robotic / automated sample preparation systems? ZipTipm-C18 compatibility has been demonstarted with the following robotic / automated sample preparation systems:

    1. ABI Symbiot™ Sample Workstation for MALDI TOF Biospectrometry™ with Universal Cone for ZipTip (cat. no. GEN602987) and Rack Adapter for the ZipTip box.

    2. Bruker Daltonics MAP™ II and MAP™ II/8 MADLI Auto Prep Station

    3. Micromass 2700-MS and MassPREP™ Sample Preparation Stations

    4. Genomic Solutions ProMS™ Sample Preparation Station

    5. Cy-Bio CyBi™ Well 96 automated pipettor

    6. Packard BioScrences MultiPROBE

    7. Tecan GENESIS Workstation
    Can I use ZipTipC18 or ZipTipµ-C18 for amino acids? ZipTipC18 and ZipTipµ-C18exhibit comparable binding properties to C18 reversed-phase HPLC. As such, lower recoveries will be seen for hydrophilic amino acids.

    C18 is offered in two bed volumes:

    ZipTipC18 - a standard bed of 0.6 µL for sample elution in 1 to 4 µL
    ZipTipµ-C18 - a micro bed of 0.2 µL for sample elution in < 1 µL.
    Can ZipTip be reused? ZipTip is intended for single use.
    How are ZipTip pipette tips packaged? ZipTip pipette tips are available in four pack sizes:
    • 8 Pack Resealable Tray
    • 24 Pack Resealable Tray
    • 96 Pack 96 place tip rack
    • 960 Pack 10 x 96 place tip rack
    How can I elute from ZipTip pipette tips directly into a nanospray needle? We suggest cutting a gel loading tip (Eppendorf 0030 001 222) approximately 2-3 mm above where the tip widens from the capillary end. ZipTip binding, washing and elution are performed utilizing the standard protocols listed in the ZipTip Reversed-Phase Operating Manual. Prior to the final dispense (elution of the peptide or protein) the cut down gel loading tip is pressed on to the end of the ZipTip using a slight twisting motion. The friction fit is tight, allowing sample to be dispensed directly into an nanospray needle.

    Refer to the ZipTip Reversed-Phase Operators Manual for a detailed diagram demonstrating the above procedure.
    How can I improve binding of hydrophilic peptides and proteins to ZipTipC18, ZipTipµ-C18 and ZipTipC4? Insure that TFA concentration is at least 0.1- 1% with minimal sample dilution. Detergents can interfere with sample binding, (especially with hydrophilic peptides). Dilute detergent to < 0.05%. Alternatively HFBA (heptafluorobutyric acid) may be used as the ion pairing agent. In some cases the peptide may be too hydrophilic for binding to the resin.
    How can I improve salt removal with ZipTipC18, ZipTipµ-C18 or ZipTipC4? Wash ZipTip with 3 to 5 aspirate/dispense cycles of 0.1% TFA (10 ml). If salt contamination persists, consider the following: For Peptides - Try washing peptides with 10% ACN (acetonitrile)/0.1% TFA. For proteins, the above ACN concentration can be increased to 30% Add 100 microliters of additional wash solution to a pipet and slowly push this solution through the Zip Tip. If the problem persists try blotting the Zip Tip on a paper towel to remove the residual wash soulution remainig in the Zip Tip after dispensing the final wash.
    How do I process sample volumes larger than 10 ml through a ZipTip? The entire volume of a sample should be passed through the ZipTip 5 times for sufficient binding. a ZipTip may be utilized on a P-100, P-200, P-500 or P-1000 pipettor by press fitting the ZipTip onto the end of an appropriate pipette tip. Please refer to ZipTip page on the Millipore WEB site.
    How many times do I have to cycle the sample through ZipTip to achieve binding to the resin? The ZipTip Operators Manual recommends to aspirate and dispense samples 3 to 7 cycles for simple mixtures and up to 10 cycles for complex mixtures to achieve maximum binding.
    How quickly can I desalt and concentrate peptide, protein or oligonucleotide samples with ZipTip pipette tips? ZipTip concentrates, and desalts samples in about one minute.

    User Guides

    Title
    ZipTip SCX Pipette Tips