Key Specifications Table
|Empirical Formula||CAS #|
|C₁₄H₂₁N₃O · 2HCl · H₂O||146986-50-7|
Pricing & Availability
An invalid quantity was specified. The product quantity has been adjusted.
|688000-100MG||Plastic ampoule||100 mg||
|688000-10MG||Glass bottle||10 mg||
|688000-1MG||Plastic ampoule||1 mg||
|688000-5MG||Glass bottle||5 mg||
|Overview||A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. Pretreatment with Y-27632 reduces the risk of lung injury in rat models of sepsis. A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688001) in H2O and a 10 mM solution in DMSO (Cat. No. 688002) are also available.|
|Synonyms||ROCK Inhibitor, (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl, Rho Kinase Inhibitor VI|
|Application||Y-27632, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.|
|Primary Target||p160 Rho-associated protein kinases (ROCK)|
|Primary Target K<sub>i</sub>||140 nM for p160 Rho-associated protein kinases (ROCK)|
|Purity||≥95% by HPLC|
|Safety Information according to GHS|
|R Phrase||R: 20/21/22
Harmful by inhalation, in contact with skin and if swallowed.
|S Phrase||S: 36
Wear suitable protective clothing.
|Product Usage Statements|
|Packaged under inert gas||Packaged under inert gas|
Y-27632 - CAS 146986-50-7 - Calbiochem SDS
|Cinel, I., et al. 2012. J. Thorac . Dis. 4, 30.
Watanabe, K., et al. 2007. Nature Biotech. 25, 681.
Chitaley, K., et al. 2001. Nat. Med. 7, 119.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Narumiya, S., et al. 2000. Methods Enzymol. 325, 273.
Hirose, M., et al. 1998. J. Cell Biol. 141, 1625.
Maekawa, M., et al. 1999. Science 285, 895.
Uehata, M., et al. 1997. Nature 389, 990.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.