WB59 Western-Re-Probe™ Reagent

WB59
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      WB59-100ML
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          Plastic ampoule 100 ml
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          Description
          OverviewDesigned to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
          A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
          Catalogue NumberWB59
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationSupplied as a 5X solution.
          Kit contains5X Re-Probe solution (125 ml)
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (see comments)
          Application CommentsSufficient volume to strip 25-30 standard blots.

          ADVANTAGES
          -Immunoblotting analyzed repeatedly with multiple probes
          -Saving on hard-to-get reagents and samples
          -Correction and re-analysis of unsatisfactory Western Blots
          -Saving time spent in gel re-running and transfer processes

          PROTOCOL
          1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
          2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
          3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
          4- Block the membrane and perform immunodetection.

          Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

          PROTOCOL SUMMARY
          1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
          2. Wash the membrane 3 times in PBST or TBST buffer
          3. Block membrane and perform immunodetection
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Kit contains5X Re-Probe solution (125 ml)
          Specifications

          Documentation

          Western-Re-Probe™ Reagent SDS

          Title

          Safety Data Sheet (SDS) 

          Western-Re-Probe™ Reagent Certificates of Analysis

          TitleLot Number
          WB59

          Technical Info

          Title
          Western Blotting Quick Reference Product Guide
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-July-2009 RFH
          ApplicationImmunoblotting (see comments)
          DescriptionWestern-Re-Probe™ Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
          FormLiquid
          FormulationSupplied as a 5X solution.
          CommentsSufficient volume to strip 25-30 standard blots.

          ADVANTAGES
          -Immunoblotting analyzed repeatedly with multiple probes
          -Saving on hard-to-get reagents and samples
          -Correction and re-analysis of unsatisfactory Western Blots
          -Saving time spent in gel re-running and transfer processes

          PROTOCOL
          1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
          2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
          3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
          4- Block the membrane and perform immunodetection.

          Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

          PROTOCOL SUMMARY
          1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
          2. Wash the membrane 3 times in PBST or TBST buffer
          3. Block membrane and perform immunodetection
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Toxicity Standard Handling