A part of MilliporeSigma

01-185 | VEGF Protein, Human recombinant

10 µg  
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      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSource
      CULT Human Recombinant human DNA expressed in baculovirus infected Sf9 cells
      Catalogue Number01-185
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionVEGF Protein, Human recombinant
      Product Information
      Presentation10 µg of VEGF lyophilized protein from buffer containing 25mM HEPES and 150mM NaCl, pH 7.0. Carrier free.
      ApplicationThe human recombinant VEGF Protein is available in a 10 µg format.
      Key Applications
      • Cell Culture
      Application NotesVEGF Proliferation Assay

      1. Prior to use in this assay, maintain HUVE cells in F-12K medium supplemented with 10% (v/v) FBS, 1% penicillin-streptomycin-glutamine, 0.1 mg/ml heparin and 0.05 mg/ml endothelial cell growth supplement (ECGS).

      2. For assay of VEGF, subculture HUVE cells into a 96-well plate at a density of 4,000 cells per well in the growth medium over night.

      3. Add VEGF to a final concentration range of 0.2, 0.4, 0.8, 1.6, 3.2, 6.2, 12.5, 25 and 50ng/ml. Add VEGF diluent to one set of wells as the negative control.
      4. Four days later, add 20ul of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well.
      5. Incubate at 37C for 2 hrs and read at OD 490nm.
      Biological Information
      SourceRecombinant human DNA expressed in baculovirus infected Sf9 cells
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
      Gene Symbol
      • VEGFA
      • VEGF-A
      • VEGF
      • VPF
      • MGC70609
      Purification MethodSterilized through a 0.2 micron membrane filter
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
      SIZE: 232 amino acids; 27042 Da
      SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
      SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
      TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
      SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.
      Stem Cell Type
      • Neural Stem Cells
      • Mesenchymal Stem Cells
      • Hematopoietic Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in a 5-day growth assay using human umbilical vein endothelial cells

      Cell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. After a quick spin, reconstitute with sterile water or 25mM HEPES and 150mM NaCl, pH 7.0. Reconstituted material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.

      This product is acid stable. Dilute in either 0.1% TFA or 4mM HCl, containing 25ug BSA per ug VEGF to maintain stability. Reducing reagents (such as dithiothrietol or 2-mercaptoethanol) inhibit VEGF activity.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      VEGF, Human Recombinant (recombinant protein expressed in Sf9 cells) - 2301935 2301935
      VEGF, Human Recombinant (recombinant protein expressed in Sf9 cells) - 2394233 2394233
      VEGF, Human Recombinant (recombinant protein expressed in Sf9 - JBC1873470 JBC1873470
      VEGF, Human Recombinant - 1627932 1627932
      VEGF, Human Recombinant - 2035500 2035500
      VEGF, Human Recombinant - DAM1724574 DAM1724574
      VEGF, Human Recombinant - DAM1776535 DAM1776535
      VEGF, Human Recombinant - R0803D0007 R0803D0007
      VEGF, Human Recombinant -2635707 2635707
      VEGF, Human Recombinant -2645279 2645279
      VEGF, Human Recombinant -2645279A 2645279A
      VEGF, Human Recombinant -2676574 2676574
      VEGF, Human Recombinant -2676574A 2676574A
      VEGF, Human Recombinant -2686873A 2686873A
      VEGF, Human Recombinant -2694453 2694453
      VEGF, Human Recombinant -2707870 2707870
      VEGF, Human Recombinant -2707870A 2707870A
      VEGF, Human Recombinant -2807919 2807919
      VEGF, recombinant - 16286 16286
      VEGF, recombinant - 17097 17097
      VEGF, recombinant - 17688 17688
      VEGF, recombinant - 20599 20599
      VEGF, recombinant - 20599a 20599a
      VEGF, recombinant - 21477U 21477U
      VEGF, recombinant - 21573U 21573U
      VEGF, recombinant - 23590U 23590U
      VEGF, recombinant - 25955U 25955U


      Reference overviewApplicationPub Med ID
      Vascular endothelial growth factor stimulates tyrosine phosphorylation and recruitment to new focal adhesions of focal adhesion kinase and paxillin in endothelial cells
      Abedi, H. and Zachary, I.
      J Biol Chem, 272:15442-51 (1997) 1997

      Cell Culture9182576 9182576
      Vascular endothelial cell growth factor (VEGF) produced by A-431 human epidermoid carcinoma cells and identification of VEGF membrane binding sites.
      Myoken, Y, et al.
      Proc. Natl. Acad. Sci. U.S.A., 88: 5819-23 (1991) 1991

      Show Abstract
      1712106 1712106

      Data Sheet

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      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors