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14-857 | Ubiquitin Activating Enzyme E1

50 µg  
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      Replacement Information

      Key Specifications Table

      Entrez Gene NumberSpeciesUni Prot Number
      NM_003334.2, NM_153280.1 Human P22314
      Catalogue Number14-857
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbiquitin Activating Enzyme E1
      OverviewFull length recombinant human E1 was expressed in E.coli and subsequently purified using nickel chelation chromatography. Ubiquitin-activating enzyme E1 is responsible for the first step in ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating with ATP the C-terminal glycine carboxyl group of ubiquitin and linking this residue to the sulphydryl side chain moiety of a cysteine residue in E1 by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
      Product Information
      Biological Information
      SourceRecombinant human ubiquitin activating enzyme E1 expressed in E. coli.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
      Gene Symbol
      • UBE1
      • MGC4781
      • A1S9T
      • A1S9
      • A1ST
      • UBE1X
      • GXP1
      Purification MethodNickel chelation chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22314 # Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
      SIZE: 1058 amino acids; 117849 Da
      SUBUNIT: Monomer (By similarity).
      SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family.
      MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
      Molecular Weight118 kDA
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaitain at -80°C for up to 12 months from date of receipt. Do not subject to repeated freeze-thaw.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Ubiquitin Activating Enzyme E1 - 0611046111 0611046111
      Ubiquitin Activating Enzyme E1 - 2010078 2010078
      Ubiquitin Activating Enzyme E1 - DAM1734779 DAM1734779
      Ubiquitin Activating Enzyme E1 - DAM1831414 DAM1831414
      Ubiquitin Activating Enzyme E1 - NG1831414 NG1831414
      Ubiquitin Activating Enzyme E1 - NG1942992 NG1942992


      Reference overviewPub Med ID
      Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation.
      Haas, A L, et al.
      J. Biol. Chem., 257: 2543-8 (1982) 1982

      Show Abstract
      6277905 6277905
      Activation of the heat-stable polypeptide of the ATP-dependent proteolytic system
      Ciechanover, A, et al
      Proc Natl Acad Sci USA, 78:761-5 (1981) 1981

      6262770 6262770


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