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662065 | Ubiquitin, Methylated, Human, Recombinant

662065
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      662065-1MG
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 1 mg
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          Description
          OverviewRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
          Catalogue Number662065
          Brand Family Calbiochem®
          SynonymsMe-Ub
          References
          ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
          Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
          Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
          Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
          Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
          Product Information
          FormLyophilized solid
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          662065

          References

          Reference overview
          Hershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
          Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
          Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
          Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
          Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.

          Brochure

          Title
          Calbiochem Biologics 32.1
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-September-2008 RFH
          SynonymsMe-Ub
          DescriptionRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
          FormLyophilized solid
          Recommended reaction conditionsTypical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
          Purity≥95% by SDS-PAGE
          SolubilityAqueous buffers (50 mg/ml)
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
          Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
          Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
          Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
          Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.