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14-871 | UbcH5b Protein, recombinant, 100 µg

100 µg  
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      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      ACT NM_003339.2, NM_181838.1 Human P62837
      Catalogue Number14-871
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbcH5b Protein, recombinant, 100 µg
      OverviewRecombinant fusion protein containing human UbcH5b with an N-terminal His-tag, expressed in E. coli.
      Product Information
      Presentation100 µg recombinant UbcH5b in 100 µL of 50mM Tris-HCl, pH 7.5, 270mM Sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 0.2mM PMSF, and 1mM Benzamidine. Frozen solution.
      ApplicationRecombinant fusion protein containing human UbcH5b with an N-terminal His-tag, expressed in E. coli. For use in Activity Assays.
      Key Applications
      • Activity Assay
      Biological Information
      Purity~85% by SDS PAGE and Coomassie blue staining.
      SourceExpressed in E. coli.
      Entrez Gene Number
      Entrez Gene SummaryThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
      Gene Symbol
      • UBE2D2
      • UBCH5B
      • PUBC1
      • E2
      • UbcH5B
      • UBC4/5
      • E2(17)KB2
      • UBC4
      Purification MethodProBond Resin chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62837 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
      SIZE: 147 amino acids; 16735 Da
      SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
      SIMILARITY: SwissProt: P62837 ## Belongs to the ubiquitin-conjugating enzyme family.
      Molecular Weight20 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by ubiquitin assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -70°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information