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GF027 | Tumor Necrosis Factor-α Protein, Recombinant mouse

GF027
20 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Mouse E.coli Greater than 98% by SDS-PAGE analysis and HPLC ana
      Description
      Catalogue NumberGF027
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTumor Necrosis Factor-α Protein, Recombinant mouse
      OverviewTumor Necrosis Factor-a (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Mouse TNF-alpha is a 17.5 kDa protein containing 156 amino acid residues.
      Alternate Names
      • TNF-alpha
      References
      Product Information
      PresentationThe sterile filtered solution was lyophilized from 5 mM Tris, pH 8.0. For reconstitution, we recommend a quick spin followed by reconstitution in sterile water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
      Applications
      ApplicationTumor Necrosis Factor-a (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, mouse TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL. Responding cells are (partial list): Tumor cells, certain other cells.
      Biological Information
      PurityGreater than 98% by SDS-PAGE analysis and HPLC ana
      SourceE.coli
      Specific ActivityThe ED(50) as determined by the cytolysis of L929 cells in the presence of actinomycin-D is <= 0.1 ng/mL, corresponding to a specific activity of >= 1x10(E7) units/mg.
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 100 ng/uL. This solution can be diluted into other buffered solutions. Reconstituted mouse TNF-alpha should be stored in undiluted aliquots at -20°C for up to 6 months.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RECOMBINANT MOUSE TUMOR NECRORSIS FACTOR-α -2677882 2677882
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2015964 2015964
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2033639 2033639
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2043872 2043872
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2080264 2080264
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2167125 2167125
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2228092 2228092
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a - 2287101 2287101
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-a -2842980 2842980
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α 2470387
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α - 2383475 2383475
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2603405 2603405
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2610962 2610962
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2718350 2718350
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2746560 2746560
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2766497 2766497
      RECOMBINANT MOUSE TUMOR NECROSIS FACTOR-α -2776849 2776849

      Data Sheet

      Title
      Tumor Necrosis Factor-alpha, recombinant mouse - Data Sheet

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors