616376 TRAIL, Human, Recombinant, NSO cells

616376
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616376-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
          Catalogue Number616376
          Brand Family Calbiochem®
          SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
          References
          ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
          Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
          Pan, G., et al. 1997. Science 277, 815.
          Pan, G., et al. 1997. Science 276, 111.
          Sheridan, J.P., et al. 1997. Science 277, 818.
          Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
          Product Information
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
          Applications
          Biological Information
          Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1 EU/µg TRAIL
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TRAIL, Human, Recombinant, NSO cells SDS

          Title

          Safety Data Sheet (SDS) 

          TRAIL, Human, Recombinant, NSO cells Certificates of Analysis

          TitleLot Number
          616376

          References

          Reference overview
          Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
          Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
          Pan, G., et al. 1997. Science 277, 815.
          Pan, G., et al. 1997. Science 276, 111.
          Sheridan, J.P., et al. 1997. Science 277, 818.
          Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.

          Citations

          Title
        • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
        • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-September-2008 RFH
          SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
          DescriptionRecombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1 EU/µg TRAIL
          Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
          SolubilityReconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
          Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
          Pan, G., et al. 1997. Science 277, 815.
          Pan, G., et al. 1997. Science 276, 111.
          Sheridan, J.P., et al. 1997. Science 277, 818.
          Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
          Citation
        • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
        • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.