A part of MilliporeSigma

21-204 | TOPglow (TCF reporter plasmid)

5 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue Number21-204
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionTOPglow (TCF reporter plasmid)
      Product Information
      ApplicationTransfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box & Luciferase open reading frame.
      Key Applications
      • Transfection
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.
      Gene Symbol
      • TCF1
      • MODY3
      • HNF1
      • HNF-1A
      • TCF-1
      • HNF1A
      • LFB1
      • HNF1a
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
      SIZE: 631 amino acids; 67356 Da
      SUBUNIT: Binds DNA as a dimer.
      DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].
      SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      TOPglow (TCF reporter plasmid) (E1B TATA-luciferase reporter) - 2448044 2448044
      TOPglow (TCF reporter plasmid) - 24574 24574
      TOPglow (TCF reporter plasmid) - 31879 31879
      TOPglow (TCF reporter plasmid) - DAM1524355 DAM1524355
      TOPglow (TCF reporter plasmid) - DAM1724580 DAM1724580


      Reference overviewPub Med ID
      Msx2 exerts bone anabolism via canonical Wnt signaling.
      Su-Li Cheng,Jian-Su Shao,Jun Cai,Oscar L Sierra,Dwight A Towler
      The Journal of biological chemistry 283 2008

      Show Abstract
      18487199 18487199
      The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
      Ishitani, T, et al.
      Nature, 399: 798-802 (1999) 1999

      Show Abstract
      10391247 10391247
      Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
      Korinek, V, et al.
      Mol. Cell. Biol., 18: 1248-56 (1998) 1998

      Show Abstract
      9488439 9488439