654205 TNF-α, Human, Recombinant, E. coli

654205
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      654205-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
          Catalogue Number654205
          Brand Family Calbiochem®
          SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
          References
          References Index 13, 9882.
          Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
          Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
          Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
          Koide, M., et al. 1993. FEBS Lett. 318, 213.
          Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
          Product Information
          CAS number94948-59-1
          FormLyophilized
          FormulationCarrier-free
          Applications
          Biological Information
          Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSMT6435000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TNF-α, Human, Recombinant, E. coli SDS

          Title

          Safety Data Sheet (SDS) 

          TNF-α, Human, Recombinant, E. coli Certificates of Analysis

          TitleLot Number
          654205

          References

          Reference overview
          Merck Index 13, 9882.
          Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
          Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
          Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
          Koide, M., et al. 1993. FEBS Lett. 318, 213.
          Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.

          Brochure

          Title
          MAPK Pathway Poster ( 750 KB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-January-2009 JSW
          SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
          DescriptionRecombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
          FormLyophilized
          FormulationCarrier-free
          CAS number94948-59-1
          RTECSMT6435000
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
          Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
          SolubilityReconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
          Toxicity Standard Handling
          References Index 13, 9882.
          Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
          Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
          Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
          Koide, M., et al. 1993. FEBS Lett. 318, 213.
          Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.

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          Life Science Research > Proteins and Enzymes > Other Proteins