616461 TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem

616461
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₂H₁₆N₄O₃. 2H₂O 301836-41-9

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616461-5MG
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          5 mg
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          Description
          OverviewA cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC50 ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220). Also available as a 100 mM solution in DMSO (Cat. No. 616464).
          Catalogue Number616461
          Brand Family Calbiochem®
          Synonyms4-[4-(3,4-Methylenedioxyphenyl)-5-(2-pyridyl)-1H-imidazol-2-yl]benzamide, Dihydrate, 4-[4-(1,3-Benzodioxol-5-yl)-5-(2-pyridyl)-1H-imidazol-2-yl]benzamide, Dihydrate, SB-431542
          References
          ReferencesIkushima, H., et al. 2009. Cell Stem Cell 5, 504.
          Lin, T., et al. 2009. Nat. Methods 6, 805.
          Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
          Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
          Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
          Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.
          Product Information
          CAS number301836-41-9
          FormOff-white solid
          Hill FormulaC₂₂H₁₆N₄O₃. 2H₂O
          Chemical formulaC₂₂H₁₆N₄O₃. 2H₂O
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥97% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          616461

          References

          Reference overview
          Ikushima, H., et al. 2009. Cell Stem Cell 5, 504.
          Lin, T., et al. 2009. Nat. Methods 6, 805.
          Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
          Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
          Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
          Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision30-August-2010 RFH
          Synonyms4-[4-(3,4-Methylenedioxyphenyl)-5-(2-pyridyl)-1H-imidazol-2-yl]benzamide, Dihydrate, 4-[4-(1,3-Benzodioxol-5-yl)-5-(2-pyridyl)-1H-imidazol-2-yl]benzamide, Dihydrate, SB-431542
          DescriptionA cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC50 ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220).
          FormOff-white solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number301836-41-9
          Chemical formulaC₂₂H₁₆N₄O₃. 2H₂O
          Structure formulaStructure formula
          Purity≥97% by HPLC
          SolubilityDMSO (100 mg/ml) or Ethanol (10 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesIkushima, H., et al. 2009. Cell Stem Cell 5, 504.
          Lin, T., et al. 2009. Nat. Methods 6, 805.
          Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
          Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
          Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
          Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.