616450 TGF-β1, Human Platelets

616450
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥97% by SDS-PAGE

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616450-1UG
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          Glass bottle 1 μg
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          Description
          OverviewNative transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigeminal neurinomal cells.
          Catalogue Number616450
          Brand Family Calbiochem®
          SynonymshTGF-β1, Transforming Growth Factor-β1
          References
          ReferencesLomo, J., et al. 1995. J. Immunol. 154, 1634.
          Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
          Bermundez, L.E. 1993. J. Immunol. 150, 1838.
          Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
          Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
          Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.
          Product Information
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution of 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.
          Applications
          Biological Information
          Biological activityED₅₀ = 40-10 pg/ml as measured by the inhibition of IL-4-dependant proliferation of mouse HT-2 cells using resazurin dye as an indicator of cell proliferation.
          Purity≥97% by SDS-PAGE
          SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Physicochemical Information
          ContaminantsEndotoxin: ≤1 EU/ug of TGF-β1
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C) for short-term storage or freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 months at 4°C or up to 3 months at -20°C..
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TGF-β1, Human Platelets SDS

          Title

          Safety Data Sheet (SDS) 

          TGF-β1, Human Platelets Certificates of Analysis

          TitleLot Number
          616450

          References

          Reference overview
          Lomo, J., et al. 1995. J. Immunol. 154, 1634.
          Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
          Bermundez, L.E. 1993. J. Immunol. 150, 1838.
          Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
          Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
          Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.

          Brochure

          Title
          TGF-b Signaling: Dual Role in Tumor Suppression and Oncogenesis (EMD)
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-May-2008 RFH
          SynonymshTGF-β1, Transforming Growth Factor-β1
          DescriptionNative transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigerminal neurinomal cells.
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution of 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.
          SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1 EU/ug of TGF-β1
          Biological activityED₅₀ = 40-10 pg/ml as measured by the inhibition of IL-4-dependant proliferation of mouse HT-2 cells using resazurin dye as an indicator of cell proliferation.
          SolubilityReconstitute to a concentration ≥10 µg/ml in sterile 4 mM HCl containing ≥0.1% HSA or BSA.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C) for short-term storage or freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 months at 4°C or up to 3 months at -20°C..
          Toxicity Standard Handling
          Merck USA index14, 9572
          ReferencesLomo, J., et al. 1995. J. Immunol. 154, 1634.
          Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
          Bermundez, L.E. 1993. J. Immunol. 150, 1838.
          Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
          Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
          Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.

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          Categories

          Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors