69048 T7•Tag® Antibody HRP Conjugate

69048
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      69048-3
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          Plastic ampoule 100 ul
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          Description
          OverviewThe T7•Tag Antibody HRP Conjugate is useful as a single detection reagent and eliminates the need for secondary antibodies or streptavidin conjugates.The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

          The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

          This antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase.

          SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
          Species/IsotypeMouse monoclonal IgG2b
          Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
          Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
          < 1 ng (developed with chemiluminescent substrates)
          FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
          Working dilution1:5,000–1:10,000
          Catalogue Number69048
          Brand Family Novagen®
          References
          Product Information
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          Biological Information
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          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          Supplemental Information
          Specifications

          Documentation

          T7•Tag® Antibody HRP Conjugate SDS

          Title

          Safety Data Sheet (SDS) 

          T7•Tag® Antibody HRP Conjugate Certificates of Analysis

          TitleLot Number
          69048

          Brochure

          Title
          Protein Purification and Detection Tools

          Citations

          Title
        • Niharika B. Mettu, et al. (2007) The nuclear receptor-coactivator interaction surface as a target for peptide antagonists of the peroxisome proliferator-activated receptors. Molecular Endocrinology 21, 2361-2377.
        • Bryan M. Wittmann, Andrea Sherk and Donald P. McDonnell. (2007) Definition of functionally important mechanistic differences among selective estrogen receptor down-regulators. Cancer Research 67, 9549-9560.
        • Debaditya Mukhopadhyay, et al. (2006) SUSP1 antagonizes formation of highly SUMO2/3-conjugated species. Journal of Cell Biology 174, 939-949.
        • Pierre Fotso, et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric golgi (COG) complex. Journal of Biological Chemistry 280, 27613-27623.
        • Erika Isono, et al. (2005) Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry 280, 6537-6547.
        • Yoshimitsu Takahashi and Yoshiko Kikuchi. (2005) Yeast PIAS-type Ull1/Siz1 is composed of SUMO ligase and regulatory domains. Journal of Biological Chemistry 280, 35822-35828.
        • Naohide Watanabe and Eric Lam. (2005) Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast. Journal of Biological Chemistry 280, 14691-14699.
        • Taruho S. Kuroda, Hiroyoshi Ariga and Mitsunori Fukuda. (2003) The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes. Molecular and Cellular Biology 23, 5245-5255.
        • Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry 276, 23992-23999.
        • User Protocols

          Title
          TB137 T7•Tag® Antibody HRP Conjugate

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies