70014 T7Select® Packaging Kit

70014
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70014-3
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          Glass bottle 6 ea
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          Description
          OverviewNovagen's T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage). Phage assembly takes place in the E. coli cytoplasm and mature phage are released by cell lysis. Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly.

          T7Select Packaging Extracts are optimized for maximum packaging efficiencies of T7Select vectors ligated with insert. The high efficiency extracts (> 2 x 108 pfu/µg with T7Select Packaging Control DNA) make it easy to produce a library containing at least 5 x 107 recombinants per microgram of arms. The extracts are made from a specially engineered phage that reduces the nonrecombinant cloning background to below 0.1%.

          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number70014
          Brand Family Novagen®
          References
          Product Information
          6T7 Packaging Extracts
          1 µgT7Select Packaging Control DNA
          0.2 mlBL21 glycerol stock
          0.2 mlBLT5403 glycerol stock
          0.2 mlBLT5615 glycerol stock
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          T7Select® Packaging Kit SDS

          Title

          Safety Data Sheet (SDS) 

          T7Select® Packaging Kit Certificates of Analysis

          TitleLot Number
          70014

          Citations

          Title
        • Rotem Edgar, et al. (2006) High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes. Proceedings of the National Academy of Sciences (USA) 103, 4841-4845.
        • Lauren R.H. Krumpe, et al. (2006) T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries. Proteomics 6, 4210-4222.
        • Felicity K. Kerr, et al. (2005) Elucidation of the substrate specificity of the C1s protease of the classical complement pathway. Journal of Biological Chemistry 280, 39510-39514.
        • Lianglin Zhang, Jason A. Hoffman and Erkki Ruoslahti. (2005) Molecular profiling of heart endothelial cells. Circulation Research 112, 1601-1611.
        • Zhaozhong Han, et al. (2002) Discovery of a stable dimeric mutant of cyanovirin-N (CV-N) from a T7 phage-displayed CV-N mutant library. Biochemical and Biophysical Research Communications 292, 1036-1043.
        • Kimmo Porkka, et al. (2002) A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Procedings of the National Academy of Science 99, 7444-7449.
        • M.P. Burgoon, et al. (2001) Antigen discovery in chronic human inflammatory central nervous disease: panning phage-display antigen libraries identifies the targets of central nervous system-derived IgG in subacute sclerosing panencephalitis. Journal of Immunology 167, 6009-6014.
        • Stefan Danner and Joel G. Belasco. (2001) T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Procedings of the National Academy of Science 98, 12954-12959.
        • Valerie A Fadok, et al. (2000) A receptor for phosphatidylserine-specific clearance of apoptotic cells. 405, 85-90.
        • Huiming Guo, et al. (2000) EMMPRIN (CD147), an inducer of matrix metalloproteinase synthesis, also binds interstitial collagenase to the tumor cell surface. Cancer Research 60, 888-891.
        • Hamid Houshmand, Gunnar Froman and Goran Magnusson. (1999) Use of bacteriophage T7 displayed peptides for determination of monoclonal antibody specificity and biosensor analysis. Analytical Biochemistry 268, 363-370.
        • Sara Nakielny, et al. (1999) Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain. European Molecular Biology Organization Journal 18, 1982-1995.
        • M. Yamamoto, Y. Kominato and F. Yamamoto. (1999) Phage display cDNA cloning of protein with carbohydrate affinity. Biochemical and Biophysical Research Communications 255, 194-199.
        • User Protocols

          Title
          TB178 T7Select® System Manual

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